| RRC ID |
41859
|
| 著者 |
Saito S, Aoki H, Ito A, Ueno S, Wada T, Mitsuzuka K, Satoh M, Arai Y, Miyagi T.
|
| タイトル |
Human alpha2,3-sialyltransferase (ST3Gal II) is a stage-specific embryonic antigen-4 synthase.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Monosialosyl globopentaosylceramide (MSGb5), originally described as stage-specific embryonic antigen-4, is expressed in testicular germ cell tumors and in aggressive cases of human renal cell carcinoma (RCC). Clarification of the molecular mechanisms regulating synthesis of MSGb5 is very important to understand testicular carcinogenesis and the malignant progression of human RCC. For this purpose, we have investigated alpha2,3-sialyltransferase involved in the synthesis of MSGb5. We used the method of expression cloning combined with polymerase chain reaction targeted to sialylmotif to isolate a cDNA clone from RCC cell line ACHN library. The cloned cDNA was found to be identical to the previously cloned ST3Gal II in sequence. A soluble recombinant form of the protein in COS-1 cells showed an enzyme activity of alpha2,3-sialyltransferase toward globopentaosylceramide (Gb5) in addition to asialo-GM1 and GM1a. Transient transfection of COS-7 and ACHN cells with this cDNA induced an increase of MSGb5, whereas stable transfection of antisense ST3Gal II cDNA suppressed expression of MSGb5 in ACHN cells. The ST3Gal II mRNA level was increased in 7 of 8 RCC cell lines and in all six RCC tissues surgically obtained, although it was not necessarily consistent with the MSGb5 level in RCC cell lines. This study indicates that ST3Gal II is a MSGb5 (stage-specific embryonic antigen-4) synthase and that its increased expression level is closely related to renal carcinogenesis.
|
| 巻・号 |
278(29)
|
| ページ |
26474-9
|
| 公開日 |
2003-7-18
|
| DOI |
10.1074/jbc.M213223200
|
| PII |
S0021-9258(20)84597-9
|
| PMID |
12716912
|
| MeSH |
Animals
Base Sequence
COS Cells
Carbohydrate Sequence
Carcinoma, Renal Cell / enzymology
Carcinoma, Renal Cell / genetics
Cloning, Molecular
DNA, Antisense / genetics
DNA, Complementary / genetics
Gangliosides / biosynthesis
Gangliosides / chemistry
Glycosphingolipids / biosynthesis*
Glycosphingolipids / chemistry
Humans
Kidney Neoplasms / enzymology
Kidney Neoplasms / genetics
Male
Molecular Sequence Data
RNA, Messenger / genetics
RNA, Messenger / metabolism
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Sialyltransferases / genetics
Sialyltransferases / metabolism*
Stage-Specific Embryonic Antigens
Testicular Neoplasms / enzymology
Testicular Neoplasms / genetics
Transfection
Tumor Cells, Cultured
beta-Galactoside alpha-2,3-Sialyltransferase
|
| IF |
4.238
|
| 引用数 |
32
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
CV-1(RCB0160) |
