論文 - 詳細
| RRC ID | 4186 |
|---|---|
| 著者 | Rose S, Malabarba MG, Krag C, Schultz A, Tsushima H, Di Fiore PP, Salcini AE. |
| タイトル | Caenorhabditis elegans intersectin: a synaptic protein regulating neurotransmission. |
| ジャーナル | Mol Biol Cell |
| Abstract |
Intersectin is a multifunctional protein that interacts with components of the endocytic and exocytic pathways, and it is also involved in the control of actin dynamics. Drosophila intersectin is required for viability, synaptic development, and synaptic vesicle recycling. Here, we report the characterization of intersectin function in Caenorhabditis elegans. Nematode intersectin (ITSN-1) is expressed in the nervous system, and it is enriched in presynaptic regions. The C. elegans intersectin gene (itsn-1) is nonessential for viability. In addition, itsn-1-null worms do not display any evident phenotype, under physiological conditions. However, they display aldicarb-hypersensitivity, compatible with a negative regulatory role of ITSN-1 on neurotransmission. ITSN-1 physically interacts with dynamin and EHS-1, two proteins involved in synaptic vesicle recycling. We have previously shown that EHS-1 is a positive modulator of synaptic vesicle recycling in the nematode, likely through modulation of dynamin or dynamin-controlled pathways. Here, we show that ITSN-1 and EHS-1 have opposite effects on aldicarb sensitivity, and on dynamin-dependent phenotypes. Thus, the sum of our results identifies dynamin, or a dynamin-controlled pathway, as a potential target for the negative regulatory role of ITSN-1. |
| 巻・号 | 18(12) |
| ページ | 5091-9 |
| 公開日 | 2007-12-1 |
| DOI | 10.1091/mbc.e07-05-0460 |
| PII | E07-05-0460 |
| PMID | 17942601 |
| PMC | PMC2096573 |
| MeSH | Adaptor Proteins, Vesicular Transport / genetics Adaptor Proteins, Vesicular Transport / metabolism* Aldicarb / pharmacology Animals Caenorhabditis elegans / drug effects Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Gene Deletion Gene Expression Regulation / genetics* Mutation / genetics Neurons / metabolism* |
| IF | 3.791 |
| 引用数 | 20 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm310 tm725 |