Reference - Detail
RRC ID | 41872 |
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Author | Suda A, Takiguchi Y, Omori S, Miyazawa H, Sugimoto T, Kurosu K, Tatsumi K, Tanabe N, Kuriyama T, Hiroshima K, Kimura H. |
Title | Gene delivery to the lung by means of syngeneic fibroblasts: an experimental model. |
Journal | Exp Lung Res |
Abstract |
An experimental model, in which exogenous gene expression in the lung is achieved, has been established. A fibroblast cell line was transfected with the lacZ gene and was administered to syngeneic mice by either intravenous or intratracheal injection. Enzyme-linked immunosorbent assay and histochemical detection of beta-galactosidase revealed efficient gene delivery to the lung by either route. Kinetic studies demonstrated that the expression peaked immediately after the injection, and this high level was maintained for 7 days by intratracheal and for 14 days by intravenous administration. This system may have potential relevance for certain experimental models requiring specific gene delivery to the lung. |
Volume | 29(4) |
Pages | 227-41 |
Published | 2003-6-1 |
DOI | 10.1080/01902140303781 |
PII | 1T6DVBP8CRFLPEQU |
PMID | 12746048 |
MeSH | Animals Cell Transplantation Female Fibroblasts / cytology Fibroblasts / enzymology* Fibroblasts / transplantation Galactosidases / genetics* Galactosidases / metabolism Gene Transfer Techniques* Gene Transfer, Horizontal* Genetic Vectors Lac Operon / genetics Lung / cytology Lung / enzymology* Mice Mice, Inbred BALB C Models, Animal Specific Pathogen-Free Organisms Transfection |
IF | 1.702 |
Times Cited | 2 |
WOS Category | RESPIRATORY SYSTEM |
Resource | |
Human and Animal Cells | BALB/3T3 clone A31(RCB0005) |