| RRC ID |
41874
|
| 著者 |
Saito Y, Yamagishi N, Ishihara K, Hatayama T.
|
| タイトル |
Identification of alpha-tubulin as an hsp105alpha-binding protein by the yeast two-hybrid system.
|
| ジャーナル |
Exp Cell Res
|
| Abstract |
Hsp105alpha is a mammalian stress protein that belongs to the HSP105/110 family. Hsp105alpha prevents stress-induced apoptosis in neuronal cells and binds to Hsp70/Hsc70 and suppresses the Hsp70 chaperone activity in vitro. In this study, to further elucidate the function of Hsp105alpha, we searched for Hsp105alpha-binding proteins by screening a mouse FM3A cell cDNA library with full-length Hsp105alpha using the yeast two-hybrid system and obtained alpha-tubulin as an Hsp105alpha-binding protein. Hsp105alpha bound directly to alpha-tubulin both in vitro and in vivo. Indirect immunofluorescence analysis with anti-Hsp105 and anti-alpha-tubulin antibodies indicated that Hsp105alpha was colocalized with microtubules. Furthermore, the disorganization of microtubules induced by heat shock was prevented in Hsp105alpha-overexpressing COS-7 cells. These findings suggested that Hsp105alpha associates with alpha-tubulin and microtubules in cells and plays a role in protection of microtubules under conditions of stress.
|
| 巻・号 |
286(2)
|
| ページ |
233-40
|
| 公開日 |
2003-6-10
|
| DOI |
10.1016/s0014-4827(03)00054-5
|
| PII |
S0014482703000545
|
| PMID |
12749852
|
| MeSH |
Animals
Apoptosis / genetics
COS Cells
DNA, Complementary / analysis
DNA, Complementary / genetics
Eukaryotic Cells / metabolism*
HSP110 Heat-Shock Proteins
HSP70 Heat-Shock Proteins / genetics
HSP70 Heat-Shock Proteins / metabolism*
HeLa Cells
Heat-Shock Response / genetics
Humans
Mice
Microtubules / metabolism*
Protein Binding / genetics
Protein Structure, Tertiary / genetics
Recovery of Function / genetics
Stress, Physiological / genetics
Stress, Physiological / metabolism*
Tubulin / genetics
Tubulin / metabolism*
Two-Hybrid System Techniques
|
| IF |
3.383
|
| 引用数 |
19
|
|
WOS 分野
|
ONCOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
COS-7(RCB0539) |
