論文 - 詳細
| RRC ID | 41874 |
|---|---|
| 著者 | Saito Y, Yamagishi N, Ishihara K, Hatayama T. |
| タイトル | Identification of alpha-tubulin as an hsp105alpha-binding protein by the yeast two-hybrid system. |
| ジャーナル | Exp Cell Res |
| Abstract |
Hsp105alpha is a mammalian stress protein that belongs to the HSP105/110 family. Hsp105alpha prevents stress-induced apoptosis in neuronal cells and binds to Hsp70/Hsc70 and suppresses the Hsp70 chaperone activity in vitro. In this study, to further elucidate the function of Hsp105alpha, we searched for Hsp105alpha-binding proteins by screening a mouse FM3A cell cDNA library with full-length Hsp105alpha using the yeast two-hybrid system and obtained alpha-tubulin as an Hsp105alpha-binding protein. Hsp105alpha bound directly to alpha-tubulin both in vitro and in vivo. Indirect immunofluorescence analysis with anti-Hsp105 and anti-alpha-tubulin antibodies indicated that Hsp105alpha was colocalized with microtubules. Furthermore, the disorganization of microtubules induced by heat shock was prevented in Hsp105alpha-overexpressing COS-7 cells. These findings suggested that Hsp105alpha associates with alpha-tubulin and microtubules in cells and plays a role in protection of microtubules under conditions of stress. |
| 巻・号 | 286(2) |
| ページ | 233-40 |
| 公開日 | 2003-6-10 |
| DOI | 10.1016/s0014-4827(03)00054-5 |
| PII | S0014482703000545 |
| PMID | 12749852 |
| MeSH | Animals Apoptosis / genetics COS Cells DNA, Complementary / analysis DNA, Complementary / genetics Eukaryotic Cells / metabolism* HSP110 Heat-Shock Proteins HSP70 Heat-Shock Proteins / genetics HSP70 Heat-Shock Proteins / metabolism* HeLa Cells Heat-Shock Response / genetics Humans Mice Microtubules / metabolism* Protein Binding / genetics Protein Structure, Tertiary / genetics Recovery of Function / genetics Stress, Physiological / genetics Stress, Physiological / metabolism* Tubulin / genetics Tubulin / metabolism* Two-Hybrid System Techniques |
| IF | 3.383 |
| 引用数 | 19 |
| WOS 分野 | ONCOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |