RRC ID 41875
著者 Yoneda S, Itoh D, Kuroda S, Kondo H, Umezawa A, Ohya K, Ohyama T, Kasugai S.
タイトル The effects of enamel matrix derivative (EMD) on osteoblastic cells in culture and bone regeneration in a rat skull defect.
ジャーナル J Periodontal Res
Abstract OBJECTIVE:Enamel matrix derivative (EMD) has been clinically used to promote periodontal tissue regeneration. The purpose of the present study is to clarify EMD affects on osteoblastic cells and bone regeneration.
MATERIALS AND METHODS:Mouse osteoblastic cells (ST2 cells and KUSA/A1 cells) are used in culture experiments. After cells were treated with EMD, cell growth was evaluated with DNA measurement, 5-bromo-2'-deoxyurydine (BrdU) incorporation assay. Measurement of alkaline phosphatase (ALP) activity and mineralized-nodule (MN) formation, Northern blotting analysis and zymography are also performed. In addition, EMD was applied to a rat skull defect and the defect was radiographically and histologically evaluated 2 weeks after the application.
RESULTS:EMD did not stimulate ST2 cell growth; however, it enhanced KUSA/A1 cell proliferation. Although EMD stimulated ALP activity in both the cells, ALP activity in KUSA/A1 cells was affected to a much greater degree. Corresponding to the increase in ALP activity, MN formation in KUSA/A1 cells was enhanced by EMD. EMD stimulated osteoblastic phenotype expression of KUSA/A1 cells such as type I collagen, osteopontin, transforming growth factor beta 1 and osteocalcin. EMD treatment also stimulated matrix metalloproteinase production in KUSA/A1 cells. Although the effects of EMD on osteoblastic cells depend on cell type, the overall effect of EMD on osteoblastic cells is stimulatory rather than inhibitory. Finally, EMD application to a rat skull defect accelerated new bone formation.
CONCLUSION:These results indicate that EMD affects osteoblastic cells and has potential as a therapeutic material for bone healing.
巻・号 38(3)
ページ 333-42
公開日 2003-6-1
DOI 10.1034/j.1600-0765.2003.00667.x
PII 667
PMID 12753373
MeSH Alkaline Phosphatase / analysis Analysis of Variance Animals Bone Diseases / drug therapy* Bone Regeneration / drug effects* Calcification, Physiologic / drug effects Cell Division / drug effects Cell Line Collagen Type I / analysis DNA / analysis Dental Enamel Proteins / pharmacology* Matrix Metalloproteinases / analysis Mice Osteoblasts / classification Osteoblasts / drug effects* Osteocalcin / analysis Osteopontin Phenotype Phosphoproteins / analysis Rats Sialoglycoproteins / analysis Skull / drug effects* Transforming Growth Factor beta / analysis Transforming Growth Factor beta1
IF 2.926
引用数 52
WOS 分野 DENTISTRY, ORAL SURGERY & MEDICINE
リソース情報
ヒト・動物細胞 ST2(RCB0224)