RRC ID 41876
著者 Osano K, Ono M.
タイトル State of histone modification in the rat Ig-beta/growth hormone locus.
ジャーナル Eur J Biochem
Abstract The state of acetylation in H3 and H4 histones and dimethylation in the H3 histone Lys4 residue were examined by chromatin immunoprecipitation (ChIP) at 11 targets in the rat Ig-beta/growth hormone locus. Marked enhancement of the acetylation of histones H3 and H4 and the dimethylation of H3 Lys4 was observed in the chromatin situated close to the promoter of an actively transcribed gene. Chromatin positioned near a cell-type-specific DNase I-hypersensitive site with enhancer activity had the same histone modifications as the active promoter. In one transcribed intron, chromatin with fewer histone modifications was found, and in another transcribed intron, chromatin with markedly enhanced modifications was found. In most cases, no appreciable difference in the acetylation of histones H3 and H4 was found at prominently enhanced targets. However, different acetylation levels of H3 and H4 were found at one target. The targets with enhanced dimethylation of the H3 Lys4 residue coincided with those with prominently enhanced acetylation of histones H3 and H4.
巻・号 270(11)
ページ 2532-9
公開日 2003-6-1
DOI 10.1046/j.1432-1033.2003.03628.x
PII 3628
PMID 12755709
MeSH Acetylation Animals Antigens, CD / metabolism* CD79 Antigens Cell Line Chromatin / metabolism Growth Hormone / metabolism* Histones / metabolism* Introns Models, Genetic Nucleosomes / metabolism Precipitin Tests Promoter Regions, Genetic Rats Reverse Transcriptase Polymerase Chain Reaction Temperature Tumor Cells, Cultured
引用数 9
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 BRL(RCB0273)