論文 - 詳細
RRC ID | 41877 |
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著者 | Sakai K, Hasegawa C, Okura M, Morikawa O, Ueyama T, Shirai Y, Sakai N, Saito N. |
タイトル | Novel variants of murine serotonin transporter mRNA and the promoter activity of its upstream site. |
ジャーナル | Neurosci Lett |
Abstract |
Three variants of murine serotonin transporter (5-HTT) mRNA, which consist of a different exon-one (exon 1a, exon 1b or exon 1c) and the same exon-two to exon-five, were identified. The promoter region for each exon 1 (p1a, p1b and p1c, respectively), ligated to pGL-3 enhancer vector, had activities significantly higher than the empty vector in all cell lines tested except p1c in PC-12, whereas the activity of p1c was significantly lower than the others. Effects of the treatment of dibutyryl-cyclic AMP, human interferon-alpha or mouse interferon-gamma have different profiles among COS-7, PC-12, C6 glioma and immortalized rat serotonergic raphe neurons, RN46A. These three promoter regions may play a role in the transcription of the 5-HTT and could offer a model of the regulation of 5-HTT production in humans and further the pathogenesis of depression. |
巻・号 | 342(3) |
ページ | 175-8 |
公開日 | 2003-5-22 |
DOI | 10.1016/s0304-3940(03)00292-1 |
PII | S0304394003002921 |
PMID | 12757893 |
MeSH | Alternative Splicing Animals Bucladesine / pharmacology Carrier Proteins / genetics* Cell Line Exons Gene Expression Regulation / drug effects Humans Immunologic Factors / pharmacology Interferon-alpha / pharmacology Luciferases / genetics Membrane Glycoproteins / genetics* Membrane Transport Proteins* Mice Molecular Sequence Data Nerve Tissue Proteins* Promoter Regions, Genetic* RNA, Messenger Rats Reverse Transcriptase Polymerase Chain Reaction / methods Serotonin Plasma Membrane Transport Proteins Transcription, Genetic* Transfection |
IF | 2.274 |
引用数 | 10 |
WOS 分野 | NEUROSCIENCES |
リソース情報 | |
ヒト・動物細胞 | COS-7(RCB0539) |