RRC ID 41877
Author Sakai K, Hasegawa C, Okura M, Morikawa O, Ueyama T, Shirai Y, Sakai N, Saito N.
Title Novel variants of murine serotonin transporter mRNA and the promoter activity of its upstream site.
Journal Neurosci Lett
Abstract Three variants of murine serotonin transporter (5-HTT) mRNA, which consist of a different exon-one (exon 1a, exon 1b or exon 1c) and the same exon-two to exon-five, were identified. The promoter region for each exon 1 (p1a, p1b and p1c, respectively), ligated to pGL-3 enhancer vector, had activities significantly higher than the empty vector in all cell lines tested except p1c in PC-12, whereas the activity of p1c was significantly lower than the others. Effects of the treatment of dibutyryl-cyclic AMP, human interferon-alpha or mouse interferon-gamma have different profiles among COS-7, PC-12, C6 glioma and immortalized rat serotonergic raphe neurons, RN46A. These three promoter regions may play a role in the transcription of the 5-HTT and could offer a model of the regulation of 5-HTT production in humans and further the pathogenesis of depression.
Volume 342(3)
Pages 175-8
Published 2003-5-22
DOI 10.1016/s0304-3940(03)00292-1
PII S0304394003002921
PMID 12757893
MeSH Alternative Splicing Animals Bucladesine / pharmacology Carrier Proteins / genetics* Cell Line Exons Gene Expression Regulation / drug effects Humans Immunologic Factors / pharmacology Interferon-alpha / pharmacology Luciferases / genetics Membrane Glycoproteins / genetics* Membrane Transport Proteins* Mice Molecular Sequence Data Nerve Tissue Proteins* Promoter Regions, Genetic* RNA, Messenger Rats Reverse Transcriptase Polymerase Chain Reaction / methods Serotonin Plasma Membrane Transport Proteins Transcription, Genetic* Transfection
IF 2.274
Times Cited 10
Human and Animal Cells COS-7(RCB0539)