論文 - 詳細
| RRC ID | 41896 |
|---|---|
| 著者 | Miles D, Athmanathan S, Thakur A, Willcox M. |
| タイトル | A novel apoptotic interaction between HSV-1 and human corneal epithelial cells. |
| ジャーナル | Curr Eye Res |
| Abstract |
PURPOSE:Herpes simplex virus type 1 (HSV-1) infects the cornea possibly causing blindness. The specific mechanisms of herpetic keratitis are unclear. We aimed to investigate whether HSV-1 would up- or down-regulate the apoptotic pathway of human corneal epithelial (HCE) cells. METHODS:HSV-1 infection of HCE and Vero cells was demonstrated (immunofluorescence) and apoptotic gene expression was quantified (ribonuclease protection assay). Caspase 8 protein activity (colorimetric assay) was quantified and compared to caspase 8 mRNA amounts from RPA experiments. The apoptotic index of HSV-1 infected HCE and Vero cells (apoptotic index = % of apoptotic cells in infected samples/mock treated samples) was obtained and compared to gene expression. RESULTS:A down-regulation in apoptotic gene expression was observed in HSV-1 infected HCE cells in contrast to Vero cells (infected and mock treated). Caspase 8 protein levels mirrored caspase 8 mRNA levels in HSV-1 infected HCE cells. The apoptotic index also supports this down-regulation. HSV-1 infected human corneal epithelial cells and Vero cells at similar rates. CONCLUSION:HSV-1 down-regulates the apoptotic pathway of human corneal epithelial cells. This down-regulation of apoptotic gene expression seems to be cell specific. Also infectivity is excluded in playing a role in regulation of the apoptotic pathway because HSV-1 replicated at similar rates in HCE and Vero cells. |
| 巻・号 | 26(3-4) |
| ページ | 165-74 |
| 公開日 | 2003-1-1 |
| DOI | 10.1076/ceyr.26.3.165.14899 |
| PMID | 12815544 |
| MeSH | Animals Apoptosis* / genetics Caspase 8 Caspase 9 Caspases / genetics Caspases / metabolism Cell Line, Transformed Chlorocebus aethiops Down-Regulation Epithelium, Corneal / pathology Epithelium, Corneal / physiopathology* Gene Expression Herpesvirus 1, Human* Humans Keratitis, Herpetic / pathology Keratitis, Herpetic / physiopathology* RNA, Messenger / metabolism Time Factors Vero Cells |
| IF | 1.754 |
| 引用数 | 15 |
| WOS 分野 | OPHTHALMOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |