論文 - 詳細
| RRC ID | 41919 |
|---|---|
| 著者 | Netzer P, Halter F, Ma TY, Hoa N, Nguyen N, Nakamura T, Tarnawski AS. |
| タイトル | Interaction of hepatocyte growth factor and non-steroidal anti-inflammatory drugs during gastric epithelial wound healing. |
| ジャーナル | Digestion |
| Abstract |
BACKGROUND/AIMS:Expression of the hepatocyte growth factor (HGF) and cyclooxygenase-2 (COX-2) is upregulated at the margins of healing gastric ulcers. We investigated in vitro the interference of HGF, the selective COX-2 inhibitor NS-398 and the nonselective COX inhibitor indomethacin with gastric epithelial wound healing and actin microfilament (actin-MF) formation. METHODS:Standardized gastric epithelial wounds, created in confluent RGM1 rat cell monolayers were treated with: HGF (10 ng/ml), NS-398 (1-100 microM) or indomethacin (0.01- 0.5 mM). The areas of re-epithelialization and cell proliferation were measured 24 h after wounding. Actin-MFs were labeled with fluorescein-conjugated phalloidin and their distribution was examined using a Nikon epifluorescence microscope. RESULTS:HGF caused a significant increase in gastric monolayer wound re-epithelialization and this was not affected by mitomycin C. Both indomethacin and NS-398 inhibited HGF-stimulated re-epithelialization, but the basal wound re-epithelialization rate and cell proliferation was only significantly inhibited by indomethacin. HGF triggered actin stress fiber formation which was inhibited by both indomethacin and NS-398, but only indomethacin interfered with actin-MF formation at the baseline condition. CONCLUSIONS:HGF significantly increased gastric wound re-epithelialization by activating cell migration which may be mediated by the COX-2 pathway. |
| 巻・号 | 67(3) |
| ページ | 118-28 |
| 公開日 | 2003-1-1 |
| DOI | 10.1159/000071291 |
| PII | 71291 |
| PMID | 12853723 |
| MeSH | Actins / drug effects* Animals Anti-Inflammatory Agents, Non-Steroidal / pharmacology* Cell Culture Techniques Cell Division / drug effects Cell Survival / drug effects Gastric Mucosa / drug effects* Growth Substances / pharmacology* Hepatocyte Growth Factor / pharmacology* Indomethacin / pharmacology* Models, Animal Nitrobenzenes / pharmacology* Rats Stomach Ulcer / physiopathology Sulfonamides / pharmacology* Wound Healing / drug effects* |
| IF | 2.692 |
| 引用数 | 7 |
| WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | RGM1(RCB0876) |