RRC ID 4193
Author Wragg RT, Hapiak V, Miller SB, Harris GP, Gray J, Komuniecki PR, Komuniecki RW.
Title Tyramine and octopamine independently inhibit serotonin-stimulated aversive behaviors in Caenorhabditis elegans through two novel amine receptors.
Journal J. Neurosci.
Abstract Biogenic amines modulate key behaviors in both vertebrates and invertebrates. In Caenorhabditis elegans, tyramine (TA) and octopamine (OA) inhibit aversive responses to 100%, but not dilute (30%) octanol. TA and OA also abolish food- and serotonin-dependent increases in responses to dilute octanol in wild-type but not tyra-3(ok325) and f14d12.6(ok371) null animals, respectively, suggesting that TA and OA modulated responses to dilute octanol are mediated by separate, previously uncharacterized, G-protein-coupled receptors. TA and OA are high-affinity ligands for TYRA-3 and F14D12.6, respectively, based on their pharmacological characterization after heterologous expression. f14d12.6::gfp is expressed in the ASHs, the neurons responsible for sensitivity to dilute octanol, and the sra-6-dependent expression of F14D12.6 in the ASHs is sufficient to rescue OA sensitivity in f14d12.6(ok371) null animals. In contrast, tyra-3::gfp appears not to be expressed in the ASHs, but instead in other neurons, including the dopaminergic CEP/ADEs. However, although dopamine (DA) also inhibits 5-HT-dependent responses to dilute octanol, TA still inhibits in dop-2; dop-1; dop-3 animals that do not respond to DA and cat-2(tm346) and Pdat-1::ICE animals that lack significant dopaminergic signaling, suggesting that DA is not an intermediate in TA inhibition. Finally, responses to TA and OA selectively desensitize after preexposure to the amines. Our data suggest that although tyraminergic and octopaminergic signaling yield identical phenotypes in these olfactory assays, they act independently through distinct receptors to modulate the ASH-mediated locomotory circuit and that C. elegans is a useful model to study the aminergic modulation of sensory-mediated locomotory behaviors.
Volume 27(49)
Pages 13402-12
Published 2007-12-5
DOI 10.1523/JNEUROSCI.3495-07.2007
PII 27/49/13402
PMID 18057198
PMC PMC6673087
MeSH Animals Behavior, Animal / physiology* CHO Cells COS Cells Caenorhabditis elegans Caenorhabditis elegans Proteins / agonists Caenorhabditis elegans Proteins / antagonists & inhibitors Caenorhabditis elegans Proteins / physiology* Chlorocebus aethiops Cricetinae Cricetulus Humans Mice NIH 3T3 Cells Octopamine / pharmacology Octopamine / physiology* Phylogeny Receptors, Biogenic Amine / agonists Receptors, Biogenic Amine / antagonists & inhibitors Receptors, Biogenic Amine / physiology* Serotonin / pharmacology Serotonin / physiology* Tyramine / pharmacology Tyramine / physiology*
Resource
C.elegans tm346 tm903 tm1846 tm1325