Reference - Detail
| RRC ID | 41958 |
|---|---|
| Author | Suzuki H, Nezaki Y, Kuno E, Sugiyama I, Mizutani A, Tsukagoshi N. |
| Title | Functional roles of the tissue inhibitor of metalloproteinase 3 (TIMP-3) during ascorbate-induced differentiation of osteoblastic MC3T3-E1 cells. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
The tissue inhibitor of the metalloproteinase-3 (TIMP-3) gene was isolated as a gene involved in the process of ascorbate-induced differentiation of mouse MC3T3-E1 cells by the differential display method. The functional roles of TIMP-3 were characterized by establishing stable cell lines, which constitutively expressed the TIMP-3 gene. The TIMP-3 transfectants produced type I collagen at the same level as that of normal cells in response to ascorbic acid 2-phosphate (AscP). However, the expression of the other osteoblastic marker proteins such as alkaline phosphatase (ALPase), osteopontin (OP), osteocalcin (OC), osteonectin (ON) and matrix metalloproteinases (MMPs) remained at a low level even in the presence of AscP. Furthermore, no mineralization of the extracellular matrix (ECM) occurred with the transfectants. Remodeling ECM through TIMPs and MMPs is concluded to be required for osteoblastic differentiation. |
| Volume | 67(8) |
| Pages | 1737-43 |
| Published | 2003-8-1 |
| DOI | 10.1271/bbb.67.1737 |
| PMID | 12951508 |
| MeSH | 3T3 Cells Alkaline Phosphatase / biosynthesis Animals Ascorbic Acid / analogs & derivatives Ascorbic Acid / pharmacology* Biomarkers / analysis Cell Differentiation / drug effects Collagen / biosynthesis Extracellular Matrix / metabolism Gelatinases / metabolism Gene Expression / drug effects Matrix Metalloproteinases / biosynthesis Mice Osteoblasts / cytology Osteoblasts / drug effects* Osteoblasts / enzymology* Osteoblasts / metabolism Osteocalcin / biosynthesis Osteonectin / biosynthesis Osteopontin RNA, Messenger / biosynthesis Sialoglycoproteins / biosynthesis Tissue Inhibitor of Metalloproteinase-3 / genetics Tissue Inhibitor of Metalloproteinase-3 / metabolism Tissue Inhibitor of Metalloproteinase-3 / physiology* Transfection Up-Regulation |
| IF | 1.516 |
| Times Cited | 10 |
| WOS Category | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Human and Animal Cells | MC3T3-E1(RCB1126) |