| RRC ID |
41961
|
| 著者 |
Nanmoku T, Takekoshi K, Isobe K, Kawakami Y, Nakai T, Okuda Y.
|
| タイトル |
Prolactin-releasing peptide stimulates catecholamine release but not proliferation in rat pheochromocytoma PC12 cells.
|
| ジャーナル |
Neurosci Lett
|
| Abstract |
We examined the effect of prolactin-releasing peptide (PrRP) on catecholamine secretion and DNA synthesis in rat pheochromocytoma PC12 cells. We initially confirmed the expression of both PrRP and its receptor in PC12 cells. PrRP31 and PrRP20 (> or =10 nM) significantly increased dopamine secretion from PC12 cells. However, PrRP20-stimulated dopamine secretion was markedly weaker than that of PrRP31. Both EDTA (extracellular Ca2+ chelator) and BAPTA-AM (intracellular Ca2+ chelator) effectively suppressed PrRP31 (100 nM)-induced dopamine secretion. PrRP31and PrRP20 (> or =1 nM) significantly induced an increase in the level of cAMP. The PKA inhibitor H89 (at 10 microM) impeded PrRP31- and PrRP20-induced dopamine secretion. Finally, we confirmed that PrRP did not affect DNA synthesis. These results indicate that PrRP may regulate catecholamine secretion but not the mitogenic effects in chromaffin cells.
|
| 巻・号 |
350(1)
|
| ページ |
33-6
|
| 公開日 |
2003-10-16
|
| DOI |
10.1016/s0304-3940(03)00836-x
|
| PII |
S030439400300836X
|
| PMID |
12962911
|
| MeSH |
Animals
Blotting, Northern
Catecholamines / analysis
Catecholamines / metabolism*
Chelating Agents / pharmacology
Cyclic AMP / analysis
Dose-Response Relationship, Drug
Drug Interactions
Edetic Acid / pharmacology
Egtazic Acid / analogs & derivatives*
Egtazic Acid / pharmacology
Enzyme Inhibitors / pharmacology
Hypothalamic Hormones / pharmacology*
Isoquinolines / pharmacology
Neuropeptides / pharmacology*
Nicotine / pharmacology
Nicotinic Agonists / pharmacology
PC12 Cells / drug effects*
PC12 Cells / metabolism
Pheochromocytoma / pathology
Prolactin-Releasing Hormone
RNA, Messenger / biosynthesis
Rats
Receptors, Neuropeptide / genetics*
Reverse Transcriptase Polymerase Chain Reaction / methods
Sulfonamides*
|
| IF |
2.274
|
| 引用数 |
9
|
|
WOS 分野
|
NEUROSCIENCES
|
| リソース情報 |
| ヒト・動物細胞 |
PC-12(RCB0009) |
