RRC ID 41961
Author Nanmoku T, Takekoshi K, Isobe K, Kawakami Y, Nakai T, Okuda Y.
Title Prolactin-releasing peptide stimulates catecholamine release but not proliferation in rat pheochromocytoma PC12 cells.
Journal Neurosci Lett
Abstract We examined the effect of prolactin-releasing peptide (PrRP) on catecholamine secretion and DNA synthesis in rat pheochromocytoma PC12 cells. We initially confirmed the expression of both PrRP and its receptor in PC12 cells. PrRP31 and PrRP20 (> or =10 nM) significantly increased dopamine secretion from PC12 cells. However, PrRP20-stimulated dopamine secretion was markedly weaker than that of PrRP31. Both EDTA (extracellular Ca2+ chelator) and BAPTA-AM (intracellular Ca2+ chelator) effectively suppressed PrRP31 (100 nM)-induced dopamine secretion. PrRP31and PrRP20 (> or =1 nM) significantly induced an increase in the level of cAMP. The PKA inhibitor H89 (at 10 microM) impeded PrRP31- and PrRP20-induced dopamine secretion. Finally, we confirmed that PrRP did not affect DNA synthesis. These results indicate that PrRP may regulate catecholamine secretion but not the mitogenic effects in chromaffin cells.
Volume 350(1)
Pages 33-6
Published 2003-10-16
DOI 10.1016/s0304-3940(03)00836-x
PII S030439400300836X
PMID 12962911
MeSH Animals Blotting, Northern Catecholamines / analysis Catecholamines / metabolism* Chelating Agents / pharmacology Cyclic AMP / analysis Dose-Response Relationship, Drug Drug Interactions Edetic Acid / pharmacology Egtazic Acid / analogs & derivatives* Egtazic Acid / pharmacology Enzyme Inhibitors / pharmacology Hypothalamic Hormones / pharmacology* Isoquinolines / pharmacology Neuropeptides / pharmacology* Nicotine / pharmacology Nicotinic Agonists / pharmacology PC12 Cells / drug effects* PC12 Cells / metabolism Pheochromocytoma / pathology Prolactin-Releasing Hormone RNA, Messenger / biosynthesis Rats Receptors, Neuropeptide / genetics* Reverse Transcriptase Polymerase Chain Reaction / methods Sulfonamides*
IF 2.274
Times Cited 9
Human and Animal Cells PC-12(RCB0009)