論文 - 詳細
| RRC ID | 41966 |
|---|---|
| 著者 | Umebayashi Y, Miyamoto Y, Wakita M, Kobayashi A, Nishisaka T. |
| タイトル | Elevation of plasma membrane permeability on laser irradiation of extracellular latex particles. |
| ジャーナル | J Biochem |
| Abstract |
In this report, we describe a laser-latex combination system that enables membrane-impermeable molecules to penetrate cell membranes. Laser light (Q-switched Nd:YAG laser, 532.5 nm) was used to irradiate a mixture of commercial latex particles (blue dyed, 1 micro m in diameter) and mouse fibrosarcoma (Meth-A) cells. After irradiation, membrane permeability was evaluated by flow cytometric assaying using propidium iodide (PI) and fluorescein diacetate (FDA). The proportion of permeabilized-resealed cells was affected by changes in the light intensity (approximately 780 mW/cm(2)), the irradiation time (approximately 240 s), and/or the particle concentration (approximately 10(9) particles/ml). The permeability persisted up to 20 min after light irradiation. Near the sites of individual particles, the permeability of the cell membrane is modified, probably due to localized temperature changes. These results suggest that this laser-induced permeabilization strategy constitutes a new means of delivering exogenous materials into living cells. |
| 巻・号 | 134(2) |
| ページ | 219-24 |
| 公開日 | 2003-8-1 |
| DOI | 10.1093/jb/mvg132 |
| PMID | 12966070 |
| MeSH | Animals Cell Line, Tumor Cell Membrane / metabolism* Cell Membrane / radiation effects* Cell Membrane Permeability / radiation effects* Extracellular Space / metabolism Extracellular Space / radiation effects Flow Cytometry Fluoresceins / pharmacokinetics Lasers Mice Microscopy, Fluorescence Microspheres* Propidium / pharmacokinetics Staining and Labeling |
| IF | 2.476 |
| 引用数 | 35 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Meth-A(RCB0464) |