論文 - 詳細
| RRC ID | 41968 |
|---|---|
| 著者 | Gruber R, Graninger W, Bobacz K, Watzek G, Erlacher L. |
| タイトル | BMP-6-induced osteogenic differentiation of mesenchymal cell lines is not modulated by sex steroids and resveratrol. |
| ジャーナル | Cytokine |
| Abstract |
Bone morphogenetic protein-6 (BMP-6) is a potent inducer of osteogenic differentiation and its expression is stimulated by 17beta-estradiol. The existence of a regulatory loop between sex steroids and BMP-6 is therefore reasonable to hypothesize. Here we determined whether the sex steroids 17beta-estradiol and dihydrotestosterone, and the phytoestrogen resveratrol can modulate BMP-6-induced alkaline phosphatase activity and osteocalcin expression. Mesenchymal cells of murine (osteoblastic MC3T3-E1 cells, preadipogenic ST2 cells, prechondrogenic ATDC5 cell) and human origin (osteosarcoma SaOS and HOS cells, primary bone marrow stromal cells) were cultured in the presence of recombinant BMP-6 under serum-free conditions. BMP-6 dose-, and time-dependently increased alkaline phosphatase activity in murine cell lines, but not in human cells. Osteocalcin expression was also increased upon stimulation with BMP-6. The presence of 17beta-estradiol, dihydrotestosterone, and resveratrol had no effect on BMP-6-induced alkaline phosphatase activity and osteocalcin expression. These data suggest that osteogenic differentiation in response to BMP-6 occurs independent of steroid hormones and resveratrol in mesenchymal cells that express basal receptor levels. |
| 巻・号 | 23(4-5) |
| ページ | 133-7 |
| 公開日 | 2003-8-21 |
| DOI | 10.1016/s1043-4666(03)00223-0 |
| PII | S1043466603002230 |
| PMID | 12967649 |
| MeSH | Alkaline Phosphatase / metabolism Animals Blotting, Northern Bone Morphogenetic Protein 6 Bone Morphogenetic Proteins / pharmacology* Cell Differentiation / drug effects* Cell Differentiation / physiology Cell Line Cell Line, Tumor / drug effects Dihydrotestosterone / pharmacology Dose-Response Relationship, Drug Estradiol / pharmacology Gene Expression Regulation / drug effects Gonadal Steroid Hormones / pharmacology* Humans Mesenchymal Stem Cells / drug effects Mesenchymal Stem Cells / metabolism Mesoderm / cytology Mesoderm / drug effects Mesoderm / metabolism Mice Osteocalcin / genetics Osteogenesis / drug effects* Osteogenesis / physiology RNA, Messenger / drug effects RNA, Messenger / genetics RNA, Messenger / metabolism Resveratrol Reverse Transcriptase Polymerase Chain Reaction Stilbenes / pharmacology* Time Factors |
| IF | 2.952 |
| 引用数 | 19 |
| WOS 分野 | IMMUNOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) ST2(RCB0224) |