Reference - Detail
RRC ID | 41971 |
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Author | Muneta Y, Kikuma R, Yoshihara K, Mori Y. |
Title | Cloning, expression, and tissue distribution of bovine interleukin-21. |
Journal | Vet Immunol Immunopathol |
Abstract |
Bovine interleukin-21 (IL-21) cDNA was cloned and sequenced from bovine peripheral blood lymphocytes (PBLs) stimulated with 10 microg/ml concanavalin A (ConA), 10 microg/ml phytohemagglutinin (PHA), and 50 ng/ml phorbol 12-myristate 13-acetate (PMA) for 48 h. The open reading frame of the bovine IL-21 cDNA is 459 bp in length and encodes 152 amino acids. The predicted amino acid sequence is 78.2 and 58.5% homologous to the human and murine IL-21 amino acid sequences, respectively. Recombinant bovine IL-21 was expressed by a baculovirus expression system. The bovine IL-21 was processed to the mature form in insect cells and secreted to the supernatant confirmed by N-terminal amino acid sequencing. The recombinant bovine mature IL-21 induced the proliferation of human IL-2-dependent cells, ILT-MAT. The mRNA expression for bovine IL-21 was observed in the spleen, but not in the brain, heart, lung, liver, and kidney. The bovine IL-21 identified in this study may provide new methods for the enhancement of innate immunity in cows. |
Volume | 95(1-2) |
Pages | 73-80 |
Published | 2003-9-15 |
DOI | 10.1016/s0165-2427(03)00106-5 |
PII | S0165242703001065 |
PMID | 12969638 |
MeSH | Amino Acid Sequence Animals Baculoviridae / genetics Base Sequence Cattle / genetics* Cattle / immunology Cell Division / immunology Cells, Cultured Cloning, Molecular DNA, Complementary / genetics Humans Interleukins / genetics* Interleukins / immunology Interleukins / metabolism Interleukins / pharmacology Molecular Sequence Data RNA, Messenger / biosynthesis RNA, Messenger / genetics Recombinant Proteins / biosynthesis Recombinant Proteins / genetics Recombinant Proteins / immunology Recombinant Proteins / pharmacology Reverse Transcriptase Polymerase Chain Reaction Sequence Alignment |
IF | 1.713 |
Times Cited | 6 |
WOS Category | VETERINARY SCIENCES IMMUNOLOGY |
Resource | |
Human and Animal Cells | COS-7(RCB0539) |