RRC ID 41974
著者 Minami T, Nakanishi Y, Izumi M, Harada T, Hara N.
タイトル Enhancement of antigen-presenting capacity and antitumor immunity of dendritic cells pulsed with autologous tumor-derived RNA in mice.
ジャーナル J Immunother
Abstract Dendritic cells (DCs) are antigen-presenting cells that play an important role in antitumor immunity. Several studies have reported that DCs pulsed with RNA from tumor cells have the ability to suppress tumors, but the details regarding the function and the immune-mechanism of DCs transfected with RNA remain to be elucidated. In this study, we investigated the transfection efficiency of RNA into DCs, and the functional modification and the antitumor efficacy of DCs pulsed with tumor-derived RNA. After the transfection of tumor-derived RNA into DCs cultured from the bone marrow of mice, pulsed DCs exhibited a high expression of both MHC antigens and CD86 on the cell surface as well as cultured DCs, and had a stronger ability both to present antigen on the MHC antigens and to stimulate T cells compared with DCs without transfection. DCs could sufficiently translate luciferase encoding RNA into luciferase proteins, and luciferase protein was expressed up to 12 hours in pulsed DCs. The DCs pulsed with tumor-derived RNA could elite a potent induction of cytotoxic T lymphocytes against autologous tumors, but not lysis against syngeneic normal cells. RNA-pulsed DCs exhibited a significant antitumor immunity in animal model. In conclusion, DCs could sufficiently uptake exogenous tumor-derived RNA, and consequently grow to be an intermediate maturate type, and induce potent T-cell stimulation and fully cause an antitumor effect in vivo. Therapy with DCs pulsed with tumor-derived RNA is sufficiently effective and safe, and thus it is considered to be clinically useful for tumor-immunotherapy.
巻・号 26(5)
ページ 420-31
公開日 2003-1-1
DOI 10.1097/00002371-200309000-00005
PMID 12973031
MeSH 3T3 Cells Animals Antigen Presentation* Bone Marrow Cells / cytology Carcinoma, Lewis Lung Cell Differentiation Cell Line, Tumor Cells, Cultured DNA-Directed RNA Polymerases / genetics Dendritic Cells / immunology* Endocytosis Female Genes, Reporter Immunotherapy, Adoptive / methods* Lymphocyte Activation Melanoma, Experimental Mice Mice, Inbred BALB C Mice, Inbred C57BL Neoplasms, Experimental / therapy* RNA, Neoplasm* T-Lymphocytes, Cytotoxic / immunology Transfection
IF 4.11
引用数 9
WOS 分野 MEDICINE, RESEARCH & EXPERIMENTAL IMMUNOLOGY ONCOLOGY
リソース情報
ヒト・動物細胞 P-815(RCB1167) BALB/3T3 clone A31(RCB0005)