Reference - Detail
| RRC ID | 42006 |
|---|---|
| Author | Ippoushi K, Azuma K, Ito H, Horie H, Higashio H. |
| Title | [6]-Gingerol inhibits nitric oxide synthesis in activated J774.1 mouse macrophages and prevents peroxynitrite-induced oxidation and nitration reactions. |
| Journal | Life Sci |
| Abstract |
Reactive nitrogen species (RNS), such as nitric oxide (NO) and its derivatives, e.g. peroxynitrite (ONOO-), have been proposed as being able to influence signal transduction and cause DNA damage, contributing to carcinogenic processes. In this study, the effect of [6]-gingerol, a pungent phenolic compound present in ginger (Zingiber officinale Roscoe), on NO synthesis in lipopolysaccharide (LPS)-activated J774.1 macrophages was tested, and the protective ability of this compound against peroxynitrite-mediated oxidation and nitration reactions were evaluated. [6]-Gingerol exhibited dose-dependent inhibition of NO production and significant reduction of inducible NO synthase (iNOS) in LPS-stimulated J774.1 cells. Moreover, [6]-gingerol effectively suppressed peroxynitrite-induced oxidation of dichlorodihydrofluorescein, oxidative single strand breaks in supercoiled pTZ 18U plasmid DNA, and formation of 3-nitrotyrosine in bovine serum albumin (BSA) and J774.1 cells. Our results indicate that [6]-gingerol is a potent inhibitor of NO synthesis and also an effective protector against peroxynitrite-mediated damage. |
| Volume | 73(26) |
| Pages | 3427-37 |
| Published | 2003-11-14 |
| DOI | 10.1016/j.lfs.2003.06.022 |
| PII | S0024320503008737 |
| PMID | 14572883 |
| MeSH | Animals Catechols Cells, Cultured DNA Damage / drug effects Dose-Response Relationship, Drug Enzyme Induction Fatty Alcohols / pharmacology* Fluoresceins / metabolism Lipopolysaccharides / pharmacology Macrophage Activation / drug effects Macrophage Activation / physiology* Macrophages / drug effects* Macrophages / enzymology Mice Nitric Oxide / biosynthesis* Nitric Oxide Synthase / antagonists & inhibitors Nitric Oxide Synthase / biosynthesis Nitric Oxide Synthase Type II Oxidation-Reduction Peroxynitrous Acid / pharmacology Zingiber officinale* |
| IF | 3.647 |
| Times Cited | 122 |
| WOS Category | PHARMACOLOGY & PHARMACY MEDICINE, RESEARCH & EXPERIMENTAL |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | J774.1(RCB0434) |