RRC ID 42007
Author Sasaki E, Okamoto Y, Yoshida K, Okamura H, Shimizu K, Nasu F, Morimoto H, Haneji T.
Title Transblot and cytochemical identification of avidin-interacting proteins in mitochondria of cultured cells.
Journal Histochem Cell Biol
Abstract Cell lysates prepared from 3T3-L1 cells were analyzed by western blotting using the avidin-biotin complex system and anti-Bax antibody. The antibody interacted with bands of proteins with estimated molecular weights of 120, 74, 72, and 25 kDa. However, only the 25-kDa band was detected with the anti-Bax antibody when the direct immunoblotting method was used. Peroxidase-conjugated avidin interacted with the 120-, 74-, and 72-kDa bands. This interaction was not limited to 3T3-L1 cells, because peroxidase-avidin also interacted with these three proteins in MC3T3-E1, YROS, Saos-2, MG63, SCCKN, and SCCTF cells although the staining intensity was different in each cell type. Avidin-peroxidase also interacted with these three proteins in the mitochondria-containing fractions prepared from 3T3-L1 cells. FITC-streptavidin was also localized in mitochondria in the cultured cells. The localization of avidin/streptavidin-interacting proteins in mitochondria was confirmed by using double staining with FITC-streptavidin and Mito-tracker.
Volume 120(4)
Pages 327-33
Published 2003-10-1
DOI 10.1007/s00418-003-0572-x
PMID 14574588
MeSH 3T3-L1 Cells / chemistry 3T3-L1 Cells / ultrastructure Animals Blotting, Western Cells, Cultured Electrophoresis, Polyacrylamide Gel Indicators and Reagents Mice Mitochondria / chemistry* Mitochondrial Proteins / analysis* Proto-Oncogene Proteins / analysis Proto-Oncogene Proteins c-bcl-2* Streptavidin* bcl-2-Associated X Protein
IF 3.418
Times Cited 4
WOS Category MICROSCOPY CELL BIOLOGY
Resource
Human and Animal Cells SCCKN(RCB0441) SCCTF(RCB0442)