RRC ID 42030
Author Lee BH, Yoshimatsu K, Maeda A, Ochiai K, Morimatsu M, Araki K, Ogino M, Morikawa S, Arikawa J.
Title Association of the nucleocapsid protein of the Seoul and Hantaan hantaviruses with small ubiquitin-like modifier-1-related molecules.
Journal Virus Res.
Abstract We performed yeast two-hybrid screening of a human kidney cell cDNA library to study the biological role of the hantavirus nucleocapsid protein (NP). We found that Seoul virus (SEOV) and Hantaan virus (HTNV) NPs were associated with small ubiquitin-like modifier (SUMO)-1-interacting proteins PIAS1, PIASxbeta, HIPK2, CHD3, and TTRAP, which interacted with the SUMO-1 conjugating enzyme (Ubc-9) and SUMO-1 in the yeast two-hybrid assay. Interactions between the HIPK2, CHD3, and TTRAP proteins and SEOV NP were also shown in a mammalian two-hybrid assay. However, there was no interaction between PIAS proteins and NP, which was probably due to the inhibitory effect of PIAS on transcription in the mammalian two-hybrid assay. Nevertheless, a co-expression experiment suggested the existence of a PIAS-NP interaction in the cytoplasm. The region spanning amino acids 100-125 of SEOV NP, which represents a critical region for NP-NP polymerization, was found to be responsible for the interaction with SUMO-1-related molecules in both the yeast and mammalian two-hybrid assays. These results add to the information on interactions of hantavirus NP and host cellular proteins.
Volume 98(1)
Pages 83-91
Published 2003-12
DOI 10.1016/j.virusres.2003.09.001
PII S0168170203002582
PMID 14609633
MeSH Animals Capsid Proteins / metabolism* Cell Line Cercopithecus aethiops Cytoplasm / metabolism Gene Library Hantaan virus / metabolism* HeLa Cells Humans Nucleocapsid Proteins / metabolism* Protein Inhibitors of Activated STAT Proteins / metabolism* SUMO-1 Protein / metabolism* Seoul virus / metabolism* Ubiquitin-Conjugating Enzymes / metabolism Vero Cells Viral Core Proteins / metabolism*
IF 2.736
Times Cited 38
Human and Animal Cells