RRC ID 42035
著者 Kato K, Umezawa K, Funeriu DP, Miyake M, Miyake J, Nagamune T.
タイトル Immobilized culture of nonadherent cells on an oleyl poly(ethylene glycol) ether-modified surface.
ジャーナル Biotechniques
Abstract Microarrays of living cells are an emerging tool in systems such as reverse transfection. These studies are limited to adherent cells partly because of the difficulty of cell immobilization. Using a newly developed reagent, the biocompatible anchor for membrane (BAM), we show herein the rapid and strong attachment of living nonadherent cells and adherent cells on BAM-modified surfaces. Normal cellular growth was observed for over 7 days on BAM-modified surfaces. We expect this methodology to greatly expand the scope of current cell microarray technology.
巻・号 35(5)
ページ 1014-8, 1020-1
公開日 2003-11-1
DOI 10.2144/03355rr01
PMID 14628675
MeSH Animals Biocompatible Materials / chemical synthesis* Biocompatible Materials / chemistry Biosensing Techniques / instrumentation Biosensing Techniques / methods Cell Adhesion / physiology* Cell Culture Techniques / methods* Cell Division / physiology* Cell Survival / physiology Cells, Immobilized / physiology* Ether / chemistry* Humans Jurkat Cells K562 Cells Membranes, Artificial Mice NIH 3T3 Cells Polyethylene Glycols / chemistry* Surface Properties
IF 1.541
引用数 61
WOS 分野 BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 NIH/3T3