| RRC ID |
42049
|
| 著者 |
Dan S, Shirakawa M, Mukai Y, Yoshida Y, Yamazaki K, Kawaguchi T, Matsuura M, Nakamura Y, Yamori T.
|
| タイトル |
Identification of candidate predictive markers of anticancer drug sensitivity using a panel of human cancer cell lines.
|
| ジャーナル |
Cancer Sci
|
| Abstract |
We previously investigated the correlations between the expression of 9216 genes and various chemosensitivities in a panel of 39 human cancer cell lines(1)) and found that the expression levels of AKR1B1 and CTSH were correlated with sensitivity and resistance to multiple drugs, respectively. To validate these correlations, we investigated the expression of these two genes and the chemosensitivities in 12 additional gastric cancer cell lines. The expression of AKR1B1 in the additional cell lines exhibited significant correlations with sensitivities to 8 of the 23 drugs examined, while that of CTSH displayed a significant negative correlation with only one (MS-247) of the 27 drugs examined. Their expressions were weakly correlated with sensitivity and resistance, respectively, to the remainder of the drugs. Moreover, when the 12 cell lines were divided into high-expressing and low-expressing groups, a comparison of these groups using Mann-Whitney's U test revealed that high expression levels of AKR1B1 and CTSH were related to sensitivity to 21 of the drugs and resistance to 8 of the drugs, respectively. The present results suggest that AKR1B1 and CTSH may be good markers for prediction of sensitivity to certain drugs and that our panel of 39 cell lines has the potential to identify candidate predictive marker genes.
|
| 巻・号 |
94(12)
|
| ページ |
1074-82
|
| 公開日 |
2003-12-1
|
| DOI |
10.1111/j.1349-7006.2003.tb01403.x
|
| PMID |
14662023
|
| MeSH |
Aged
Alcohol Oxidoreductases / biosynthesis
Alcohol Oxidoreductases / drug effects*
Alcohol Oxidoreductases / genetics
Aldehyde Reductase
Aldo-Keto Reductases
Antineoplastic Agents / pharmacology
Cathepsin H
Cathepsins / biosynthesis
Cathepsins / drug effects*
Cathepsins / genetics
Cell Line, Tumor
Cysteine Endopeptidases / biosynthesis
Cysteine Endopeptidases / drug effects*
Cysteine Endopeptidases / genetics
Drug Screening Assays, Antitumor / methods*
Female
Gene Expression / drug effects*
Humans
Immunoblotting
Male
Middle Aged
Neoplasms / drug therapy*
Neoplasms / genetics
Oligonucleotide Array Sequence Analysis
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction
|
| IF |
4.966
|
| 引用数 |
14
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
DU145(RCB2143)
PC-3(RCB2145)
GCIY(RCB0555)
GT3TKB(RCB0885)
HGC-27(RCB0500)
AZ521(RCB2087) |
