Reference - Detail
| RRC ID | 42061 |
|---|---|
| Author | Yamamoto M, Kuroiwa A. |
| Title | Hoxa-11 and Hoxa-13 are involved in repression of MyoD during limb muscle development. |
| Journal | Dev Growth Differ |
| Abstract |
Under the influence of the limb mesenchyme, Hoxa-11 is expressed in migrating and proliferating premyoblasts in the limb field and Hoxa-13 is induced in subdomains of congregated limb muscle masses. To evaluate the roles of Hoxa-11 and Hoxa-13 in myogenesis of the limb, we performed electroporation in ovo to force expression of these Hox genes in limb muscle precursors. In the presence of ectopic Hoxa-11, expression of MyoD was blocked transiently. In C2C12 myoblasts, transfection of Hoxa-11 also repressed the expression of endogenous MyoD. Forced expression of Hoxa-13 resulted in more pronounced repression of MyoD in both limb and C2C12 myoblasts. In contrast, targeted disruption of Hoxa-13 gave rise to enhanced expression of MyoD in the flexor carpi radialis muscle, a forearm muscle that normally expressed Hoxa-13. These results suggest that Hoxa-11 and Hoxa-13 are involved in the negative regulation of MyoD expression in limb muscle precursors. |
| Volume | 45(5-6) |
| Pages | 485-98 |
| Published | 2003-1-1 |
| DOI | 10.1111/j.1440-169x.2003.00715.x |
| PMID | 14706073 |
| MeSH | Animals Body Patterning Cell Line Chick Embryo Chickens Codon DNA, Complementary / metabolism Electroporation Extremities / embryology* Gene Expression Regulation, Developmental Homeodomain Proteins / physiology* Immunohistochemistry In Situ Hybridization Mice Muscles / embryology* MyoD Protein / metabolism* Protein Structure, Tertiary Time Factors Transfection |
| IF | 1.723 |
| Times Cited | 31 |
| WOS Category | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | F1000Research |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | C2C12(RCB0987) |