| RRC ID |
42082
|
| Author |
Handa O, Naito Y, Takagi T, Shimozawa M, Kokura S, Yoshida N, Matsui H, Cepinskas G, Kvietys PR, Yoshikawa T.
|
| Title |
Tumor necrosis factor-alpha-induced cytokine-induced neutrophil chemoattractant-1 (CINC-1) production by rat gastric epithelial cells: role of reactive oxygen species and nuclear factor-kappaB.
|
| Journal |
J Pharmacol Exp Ther
|
| Abstract |
Rat cytokine-induced neutrophil chemoattractant-1 (CINC-1), a counterpart of the human growth-regulated oncogene product (GRO), has been suggested to participate in neutrophil recruitment in an experimental model of gastritis in rat. However, the mechanism(s) involved in regulation of CINC-1 production by the gastric mucosa remains unclear. The aim of this study was to investigate the mechanism(s) of CINC-1 production by rat gastric mucosa in vitro. All experiments were performed using rat normal gastric mucosal cell line (RGM-1). RGM-1s were stimulated with tumor necrosis factor (TNF)-alpha, and CINC-1 mRNA levels (reverse transcription-polymerase chain reaction) and protein secretion (enzyme-linked immunosorbent assay) were assessed. The production of reactive oxygen species (ROS) and nuclear factor (NF)-kappaB activation (translocation to the nuclei) in response to TNF-alpha stimulation was evaluated using fluorescence microscopy in the presence or absence of the inhibitors of mitochondrial electron flow and NF-kappaB activation. Stimulation of RGM-1 cells with TNF-alpha resulted in an increase in intracellular oxidative stress, NF-kappaB translocation to the nuclei, and up-regulation of CINC-1 mRNA and protein, which was prevented by interfering with mitochondria-dependent ROS production and NF-kappaB activation. Taken together, these findings indicate that CINC-1, a counterpart of the human GRO, production by rat gastric epithelial cells in response to TNF-alpha stimulation is an oxidant stress-mediated and NF-kappaB-dependent event.
|
| Volume |
309(2)
|
| Pages |
670-6
|
| Published |
2004-5-1
|
| DOI |
10.1124/jpet.103.062216
|
| PII |
jpet.103.062216
|
| PMID |
14747614
|
| MeSH |
Animals
Chemokine CXCL1
Chemokines, CXC / genetics
Chemokines, CXC / metabolism*
Cytokines / pharmacology
Gastric Mucosa / drug effects*
Gastric Mucosa / metabolism
Humans
Intercellular Signaling Peptides and Proteins / genetics
Intercellular Signaling Peptides and Proteins / metabolism*
NF-kappa B / antagonists & inhibitors
NF-kappa B / metabolism*
Oxidative Stress / drug effects
RNA, Messenger / drug effects
RNA, Messenger / metabolism
Rats
Reactive Oxygen Species / metabolism*
Time Factors
Tumor Necrosis Factor-alpha / pharmacology*
|
| IF |
3.561
|
| Times Cited |
52
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
|
| Resource |
| Human and Animal Cells |
RGM1(RCB0876) |
