| RRC ID |
42086
|
| Author |
Itoh M, Shimazu A, Hirata I, Yoshida Y, Shintani H, Okazaki M.
|
| Title |
Characterization of CO3Ap-collagen sponges using X-ray high-resolution microtomography.
|
| Journal |
Biomaterials
|
| Abstract |
For reconstruction and regeneration of hard tissues, scaffold biomaterials with large size pores and high porosity are important, in addition to their roles as supporting frames. To develop a new biodegradable scaffold biomaterial, CO3Ap, which has crystallinity and a chemical composition similar to bone, was synthesized at pH 7.4 and 60 degrees C. Then, the CO3Ap was mixed with a neutralized collagen gel and the CO3Ap-collagen mixtures with different kinds of CO3Ap contents and porosity were lyophilized into sponges. Scanning electron micrography (SEM) observation of CO3Ap-collagen sponges showed favorable pores for cell invasion. Approximately 50-300 microm size pores appeared to continue through the bulk. Higher magnification of the sponge showed a better adhesion between CO3Ap crystals and collagen. X-ray high-resolution microtomography revealed a clear image of the 3D structure of the sponges. The porosity of 0, 70 and 90%(w/w) CO3Ap-collagen sponges was 79.2 +/- 2.8%, 72.6 +/- 2.4% and 48.9 +/- 6.1%, respectively. The 70%(w/w) CO3Ap-collagen sponge appeared to be the most favorable biomaterial from the viewpoint of natural bone properties. Mouse osteoblast MC3T3-E1 cells were cultured in alphaMEM with 10% FCS for 2 weeks. Hematoxylin-eosin staining confirmed osteoblast cells invaded well into the CO3Ap-collagen sponge. These sponges are expected to be used as hard tissue scaffold biomaterials for therapeutic uses.
|
| Volume |
25(13)
|
| Pages |
2577-83
|
| Published |
2004-6-1
|
| DOI |
10.1016/j.biomaterials.2003.09.071
|
| PII |
S0142961203007634
|
| PMID |
14751743
|
| MeSH |
3T3 Cells
Animals
Biocompatible Materials*
Cattle
Collagen / chemistry*
Mice
Microscopy, Electron, Scanning
Tomography, X-Ray / methods*
X-Ray Diffraction
|
| IF |
10.317
|
| Times Cited |
38
|
|
WOS Category
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126) |
