論文 - 詳細
| RRC ID | 4210 |
|---|---|
| 著者 | Batista PJ, Ruby JG, Claycomb JM, Chiang R, Fahlgren N, Kasschau KD, Chaves DA, Gu W, Vasale JJ, Duan S, Conte D Jr, Luo S, Schroth GP, Carrington JC, Bartel DP, Mello CC. |
| タイトル | PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegans. |
| ジャーナル | Mol Cell |
| Abstract |
In metazoans, Piwi-related Argonaute proteins have been linked to germline maintenance, and to a class of germline-enriched small RNAs termed piRNAs. Here we show that an abundant class of 21 nucleotide small RNAs (21U-RNAs) are expressed in the C. elegans germline, interact with the C. elegans Piwi family member PRG-1, and depend on PRG-1 activity for their accumulation. The PRG-1 protein is expressed throughout development and localizes to nuage-like structures called P granules. Although 21U-RNA loci share a conserved upstream sequence motif, the mature 21U-RNAs are not conserved and, with few exceptions, fail to exhibit complementarity or evidence for direct regulation of other expressed sequences. Our findings demonstrate that 21U-RNAs are the piRNAs of C. elegans and link this class of small RNAs and their associated Piwi Argonaute to the maintenance of temperature-dependent fertility. |
| 巻・号 | 31(1) |
| ページ | 67-78 |
| 公開日 | 2008-7-11 |
| DOI | 10.1016/j.molcel.2008.06.002 |
| PII | S1097-2765(08)00391-2 |
| PMID | 18571452 |
| PMC | PMC2570341 |
| MeSH | Animals Argonaute Proteins Base Sequence Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Fertility Gene Expression Regulation Germ Cells / cytology Germ Cells / metabolism Models, Biological Molecular Sequence Data Mutation / genetics Protein Binding RNA, Helminth / metabolism* RNA, Small Interfering / metabolism* RNA-Induced Silencing Complex Regulatory Sequences, Nucleic Acid / genetics |
| IF | 15.584 |
| 引用数 | 299 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 13 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm872 tm1094 |