| RRC ID |
42100
|
| Author |
Rawat SS, Eaton J, Gallo SA, Martin TD, Ablan S, Ratnayake S, Viard M, KewalRamani VN, Wang JM, Blumenthal R, Puri A.
|
| Title |
Functional expression of CD4, CXCR4, and CCR5 in glycosphingolipid-deficient mouse melanoma GM95 cells and susceptibility to HIV-1 envelope glycoprotein-triggered membrane fusion.
|
| Journal |
Virology
|
| Abstract |
We had previously reported that glycosphingolipids (GSL) support human immunodeficiency virus type 1 (HIV-1) entry. In this study, we further examined this issue by expressing HIV-1 receptors in GSL-deficient GM95 cells. GM95 cells expressing low levels of CD4 and CXCR4 or CCR5 did not support HIV-1 Env-mediated fusion. However, higher expression of these receptors rendered GM95 cells highly susceptible to fusion with cells expressing appropriate HIV-1 envelope glycoproteins (HIV-1 Envs). The GM95 cells exhibited a different fusion phenotype when compared with GSL(+) NIH3T3 cells bearing similar receptor levels. Fusion of GM95 targets expressing higher levels of CD4 and coreceptors occurred at 25 degrees C and was sensitive to cholesterol depletion or disruption of the cytoskeleton. In contrast, the fusion threshold of NIH3T3CD4X4/R5 targets was at >/=28 degrees C as previously reported and was insensitive to cholesterol depletion or cytoskeletal network disruption. On the basis of these observations, we propose that target membrane GSLs support HIV-1 Env-mediated fusion at low density of receptors by stabilizing receptor pools in natural targets.
|
| Volume |
318(1)
|
| Pages |
55-65
|
| Published |
2004-1-5
|
| DOI |
10.1016/j.virol.2003.08.042
|
| PII |
S0042682203006639
|
| PMID |
14972535
|
| MeSH |
Animals
CD4 Antigens / metabolism*
Gene Products, env / pharmacology*
Glycosphingolipids / deficiency*
Glycosphingolipids / metabolism
HIV-1 / pathogenicity
HIV-2 / pathogenicity
HeLa Cells
Humans
Melanoma
Membrane Fusion / drug effects*
Mice
NIH 3T3 Cells
Receptors, CCR5 / metabolism*
Receptors, CXCR4 / metabolism*
Tumor Cells, Cultured
|
| IF |
2.819
|
| Times Cited |
17
|
|
WOS Category
|
VIROLOGY
|
| Resource |
| Human and Animal Cells |
GM95(RCB1026) |
