RRC ID 42118
著者 Kanno S, Hirano S, Kayama F.
タイトル Effects of phytoestrogens and environmental estrogens on osteoblastic differentiation in MC3T3-E1 cells.
ジャーナル Toxicology
Abstract Phytoestrogens and environmental estrogens, which have in part some structural similarity to 17beta-estradiol, are reported to act as agonists/antagonists of estrogen in animals and humans. Estrogen is known to play an important role in maintaining bone mass, since the concentration of serum estrogen decreases after menopause and the estrogen deficiency results in bone loss. In this study, we report the effects of phytoestrogens (genistein, daidzein, and coumestrol) and environmental estrogens (bisphenol A (BPA), p-n-nonylphenol (NP) and bis(2-ethylhexyl)phthalate (DEHP)) on osteoblast differentiation using MC3T3-E1 cells, a mouse calvaria osteoblast-like cell line. Coumestrol (10(-10) to 10(-6)M) slightly enhanced cell proliferation, while neither the other phytoestrogens (daidzein, genistein) nor environmental estrogens increased cell proliferation. Alkaline phosphatase (ALP) activity and cellular calcium (Ca) and phosphorus (P) contents were increased by phytoestrogens and BPA; however, neither NP nor DEHP affected those osteoblastic indicators. The effects of estrogenic potency, using the cell proliferation of MCF-7 cells, an estrogen receptor (ER)-positive human breast cancer cell line, indicate that coumestrol has the highest estrogenic potency among those phytoestrogens and environmental estrogens. The estrogenic potency of NP and DEHP were lower than the others. In conclusion, phytoestrogens, such as coumestrol, genistein and daidzein, and BPA increased ALP activity and enhanced bone mineralization in MC3T3-E1 cells, suggesting that not only phytoestrogen but also BPA, an environmental estrogen, is implicated in bone metabolism.
巻・号 196(1-2)
ページ 137-45
公開日 2004-3-1
DOI 10.1016/j.tox.2003.12.002
PII S0300483X03005468
PMID 15036763
MeSH 3T3 Cells Alkaline Phosphatase / metabolism Animals Calcification, Physiologic / drug effects Calcium / metabolism Cell Differentiation / drug effects Cell Division / drug effects Cell Line Cell Line, Tumor Charcoal / chemistry Dextrans / chemistry Environmental Pollutants / toxicity* Estradiol Congeners / pharmacology* Estrogen Receptor alpha Estrogen Receptor beta Female Humans Isoflavones / pharmacology* Mice Mice, Inbred C57BL Osteoblasts / drug effects* Phosphorus / metabolism Phytoestrogens Plant Preparations / pharmacology* Receptors, Estrogen / biosynthesis Receptors, Estrogen / genetics Reverse Transcriptase Polymerase Chain Reaction
IF 4.099
引用数 76
WOS 分野 TOXICOLOGY PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)