RRC ID 42124
著者 Oyama K, Terashima M, Takagane A, Maesawa C.
タイトル Prognostic significance of peritoneal minimal residual disease in gastric cancer detected by reverse transcription-polymerase chain reaction.
ジャーナル Br J Surg
Abstract BACKGROUND:A sensitive method for detecting minimal residual disease in the peritoneal cavity by quantifying carcinoembryonic antigen (CEA) mRNA using real-time quantitative reverse transcription-polymerase chain reaction (RQ-RT-PCR) was developed. The clinical value of the method for predicting peritoneal recurrence in patients with gastric cancer was evaluated.
METHOD:A total of 195 patients with gastric cancer and 20 with asymptomatic cholecystolithiasis were included in the study. CEA mRNA expression in peritoneal washings (p-CEA mRNA) was measured by RQ-RT-PCR and normalized to glyceraldehyde-3-phosphate dehydrogenase mRNA expression. The cut-off level of p-CEA mRNA for gastric cancer was determined by examining p-CEA mRNA levels in patients with asymptomatic cholecystolithiasis.
RESULTS:Fifty-five (28.2 per cent) of the 195 patients were p-CEA mRNA positive. The rate of p-CEA mRNA positivity correlated significantly with clinicopathological factors. In 163 patients who underwent curative surgery, overall survival and disease-free survival were significantly poorer in p-CEA mRNA-positive patients than in p-CEA mRNA-negative patients (P < 0.001). Cox regression analysis revealed that only p-CEA mRNA was a significant independent prognostic factor (P = 0.034). Multivariate logistic regression analysis showed that p-CEA mRNA was a significant independent risk factor for peritoneal recurrence (P = 0.027).
CONCLUSION:These results suggest that p-CEA mRNA is a reliable prognostic factor and predictor of peritoneal recurrence in gastric cancer.
巻・号 91(4)
ページ 435-43
公開日 2004-4-1
DOI 10.1002/bjs.4455
PMID 15048743
MeSH Carcinoembryonic Antigen / analysis* DNA, Complementary / analysis Disease-Free Survival Humans Neoplasm, Residual / pathology Peritoneal Neoplasms / chemistry Peritoneal Neoplasms / diagnosis* Peritoneal Neoplasms / secondary Prognosis RNA, Messenger / analysis RNA, Neoplasm / analysis Reverse Transcriptase Polymerase Chain Reaction / methods Sensitivity and Specificity Stomach Neoplasms / pathology* Tumor Cells, Cultured
IF 5.676
引用数 33
WOS 分野 SURGERY
リソース情報
ヒト・動物細胞 MKN45(RCB1001) MKN74(RCB1002)