論文 - 詳細
| RRC ID | 42133 |
|---|---|
| 著者 | Ueshima S, Fukao H, Okada K, Matsuo O. |
| タイトル | Growth inhibition of vascular smooth muscle cells derived from urokinase receptor (u-PAR)-deficient mice in the presence of carcinoma cells. |
| ジャーナル | Thromb Res |
| Abstract |
The growth rate of vascular smooth muscle cells (VSMCs), which were derived from aorta of mice deficient in the fibrinolytic factors tissue-type plasminogen activator (t-PA(-/-)), urokinase (u-PA(-/-)), u-PA receptor (u-PAR(-/-)) and type 1 plasminogen activator inhibitor (PAI-1(-/-)), as well as wild-type (WT) mice, was investigated in the presence of mouse melanoma cells (B16). In the VSMCs cultured with a basal medium supplemented with 10% fetal calf serum (FCS), there was no difference in the growth rate among the gene-lacking VSMCs and WT VSMCs, indicating that these fibrinolytic factors were not involved in the FCS-mediated cell proliferation. On the other hand, when these VSMCs were cultured with B16 cells in either the mixed culture or a double-chamber, only u-PAR(-/-) VSMCs showed a significantly lower growth rate. In addition, these suppressive effects on u-PAR(-/-) VSMCs were also observed in the presence of B16-derived conditioned medium (B16/CM). The growth rate of all the VSMCs except u-PAR(-/-) VSMCs was increased in the presence of B16/CM. The degree of the increase in cell number was comparable to that obtained with FCS. These effects on growth activity were partially associated with the levels of mitogen-activated protein kinase (MAPK, p42/p44) activity. The findings suggest that u-PAR plays an important role in the proliferative response of VSMCs and that without u-PAR, there is no intracellular signaling for cell proliferation. |
| 巻・号 | 113(1) |
| ページ | 41-9 |
| 公開日 | 2004-1-1 |
| DOI | 10.1016/j.thromres.2004.01.008 |
| PII | S004938480400043X |
| PMID | 15081564 |
| MeSH | Animals Cell Division / drug effects Cell Division / physiology Cell Line, Tumor Cells, Cultured Coculture Techniques Culture Media / pharmacology Culture Media, Conditioned / pharmacology Melanoma, Experimental / metabolism* Melanoma, Experimental / pathology Mice Mitogen-Activated Protein Kinases / metabolism Muscle, Smooth, Vascular / cytology Muscle, Smooth, Vascular / growth & development* Muscle, Smooth, Vascular / physiology* Plasminogen Activator Inhibitor 1 / deficiency Plasminogen Activator Inhibitor 1 / physiology Receptors, Cell Surface / deficiency Receptors, Cell Surface / genetics Receptors, Cell Surface / physiology* Receptors, Urokinase Plasminogen Activator Tissue Plasminogen Activator / deficiency Tissue Plasminogen Activator / physiology Urokinase-Type Plasminogen Activator / deficiency Urokinase-Type Plasminogen Activator / physiology |
| IF | 2.869 |
| 引用数 | 2 |
| WOS 分野 | PERIPHERAL VASCULAR DISEASE HEMATOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | B16 |