RRC ID 42133
著者 Ueshima S, Fukao H, Okada K, Matsuo O.
タイトル Growth inhibition of vascular smooth muscle cells derived from urokinase receptor (u-PAR)-deficient mice in the presence of carcinoma cells.
ジャーナル Thromb Res
Abstract The growth rate of vascular smooth muscle cells (VSMCs), which were derived from aorta of mice deficient in the fibrinolytic factors tissue-type plasminogen activator (t-PA(-/-)), urokinase (u-PA(-/-)), u-PA receptor (u-PAR(-/-)) and type 1 plasminogen activator inhibitor (PAI-1(-/-)), as well as wild-type (WT) mice, was investigated in the presence of mouse melanoma cells (B16). In the VSMCs cultured with a basal medium supplemented with 10% fetal calf serum (FCS), there was no difference in the growth rate among the gene-lacking VSMCs and WT VSMCs, indicating that these fibrinolytic factors were not involved in the FCS-mediated cell proliferation. On the other hand, when these VSMCs were cultured with B16 cells in either the mixed culture or a double-chamber, only u-PAR(-/-) VSMCs showed a significantly lower growth rate. In addition, these suppressive effects on u-PAR(-/-) VSMCs were also observed in the presence of B16-derived conditioned medium (B16/CM). The growth rate of all the VSMCs except u-PAR(-/-) VSMCs was increased in the presence of B16/CM. The degree of the increase in cell number was comparable to that obtained with FCS. These effects on growth activity were partially associated with the levels of mitogen-activated protein kinase (MAPK, p42/p44) activity. The findings suggest that u-PAR plays an important role in the proliferative response of VSMCs and that without u-PAR, there is no intracellular signaling for cell proliferation.
巻・号 113(1)
ページ 41-9
公開日 2004-1-1
DOI 10.1016/j.thromres.2004.01.008
PII S004938480400043X
PMID 15081564
MeSH Animals Cell Division / drug effects Cell Division / physiology Cell Line, Tumor Cells, Cultured Coculture Techniques Culture Media / pharmacology Culture Media, Conditioned / pharmacology Melanoma, Experimental / metabolism* Melanoma, Experimental / pathology Mice Mitogen-Activated Protein Kinases / metabolism Muscle, Smooth, Vascular / cytology Muscle, Smooth, Vascular / growth & development* Muscle, Smooth, Vascular / physiology* Plasminogen Activator Inhibitor 1 / deficiency Plasminogen Activator Inhibitor 1 / physiology Receptors, Cell Surface / deficiency Receptors, Cell Surface / genetics Receptors, Cell Surface / physiology* Receptors, Urokinase Plasminogen Activator Tissue Plasminogen Activator / deficiency Tissue Plasminogen Activator / physiology Urokinase-Type Plasminogen Activator / deficiency Urokinase-Type Plasminogen Activator / physiology
IF 2.869
引用数 2
WOS 分野 PERIPHERAL VASCULAR DISEASE HEMATOLOGY
リソース情報
ヒト・動物細胞 B16