RRC ID 42138
Author Qiao S, Li W, Tsubouchi R, Murakami K, Yoshino M.
Title Role of vanilloid receptors in the capsaicin-mediated induction of iNOS in PC12 cells.
Journal Neurochem Res
Abstract The vanilloid receptor 1(VR1) is a nonselective cation channel that is activated by pungent vanilloid compound, extracellular protons, or noxious heat. mRNA of VR1 and vanilloid receptor 1-like receptor (VRL1) were expressed in PC12 cells, and only VRI mRNA was detected in glioma and A10 cell lines. VRI protein was demonstrated in PC12 cells by immunocytochemistry and Western blotting. Capsaicin (CPS), the VRI receptor agonist, led to an increase in intracellular calcium ion, and this effect was blocked by pretreatment with VR1 receptor antagonist capsazepin (CPZ). Treatment of PC12 cells with low concentration of CPS (5-50 microM) increased reactive oxygen species (ROS) production, and inducible nitric oxide synthase (iNOS) was expressed after CPS treatment for 24 h. These CPS-induced changes are inhibited by pretreatment of CPZ. These findings suggest that CPS-induced iNOS expression through the VR1 and/or VRL1-mediated pathway, and this may explain the CPS-mediated physiological and pathological effects in neuron system.
Volume 29(4)
Pages 687-93
Published 2004-4-1
DOI 10.1023/b:nere.0000018839.59457.5c
PMID 15098930
MeSH Animals Base Sequence Blotting, Western Calcium / metabolism Capsaicin / pharmacology* DNA Primers Enzyme Induction Mice Nitric Oxide Synthase / biosynthesis* Nitric Oxide Synthase Type I Nitric Oxide Synthase Type II PC12 Cells Rats Reactive Oxygen Species Receptors, Drug / genetics Receptors, Drug / physiology* Reverse Transcriptase Polymerase Chain Reaction
IF 2.782
Times Cited 19
Human and Animal Cells