論文 - 詳細
| RRC ID | 42140 |
|---|---|
| 著者 | Iwakiri K, Ghazizadeh M, Jin E, Fujiwara M, Takemura T, Takezaki S, Kawana S, Yasuoka S, Kawanami O. |
| タイトル | Human airway trypsin-like protease induces PAR-2-mediated IL-8 release in psoriasis vulgaris. |
| ジャーナル | J Invest Dermatol |
| Abstract |
Human airway trypsin-like protease (HAT), a novel serine protease in the airways, enhances cell growth and IL-8 production. The expression and role of HAT in the skin however, is unknown. Immunofluorescence staining and reverse transcription (RT)-PCR were done to know HAT production in normal and psoriatic tissues and keratinocyte cell lines. Cell growth and/or IL-8 release analyses were made by bromo-deoxyuridine (BrdU) uptake and ELISA. Psoriatic epidermis showed more extensive immunofluorescence expression of HAT, and less extensive expression of protease-activated receptor (PAR)-2. RT-PCR demonstrated a higher HAT and a lesser PAR-2 mRNA expressions in psoriatic epidermis. Normal keratinocyte and epidermoid carcinoma cell lines expressed HAT and PAR-2 mRNA, and immortalized keratinocytes (HaCaT) expressed PAR-2, but not HAT mRNA. PAR-2 was detected along the keratinocyte surface in culture and became invisible upon HAT stimulation, suggesting a process of its internalization. HAT or PAR-2 activating peptide did not enhance BrdU uptake, but induced an IL-8 release. Treatment with HAT and IL-1beta synergistically increased the effect of IL-8 release. Inhibition of PAR-2 resulted in a decreased HAT-induced IL-8 release. Thus, HAT might promote PAR-2-mediated IL-8 production to accumulate inflammatory cells in the epidermal layer of psoriasis. |
| 巻・号 | 122(4) |
| ページ | 937-44 |
| 公開日 | 2004-4-1 |
| DOI | 10.1111/j.0022-202X.2004.22415.x |
| PII | S0022-202X(15)30765-X |
| PMID | 15102084 |
| MeSH | Adult Aged Biological Transport / drug effects Biopsy Cell Division / drug effects Cells, Cultured DNA / biosynthesis Epidermis / metabolism Epidermis / pathology Female Humans Interleukin-18 / metabolism* Keratinocytes / metabolism Male Middle Aged Psoriasis / metabolism* Psoriasis / pathology RNA, Messenger / metabolism Receptor, PAR-2 / genetics Receptor, PAR-2 / metabolism* Recombinant Proteins / pharmacology Serine Endopeptidases / genetics Serine Endopeptidases / metabolism* Serine Endopeptidases / pharmacology Skin / metabolism Skin / pathology Tissue Distribution |
| IF | 7.143 |
| 引用数 | 44 |
| WOS 分野 | DERMATOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A431(RCB0202) |