| RRC ID |
42140
|
| 著者 |
Iwakiri K, Ghazizadeh M, Jin E, Fujiwara M, Takemura T, Takezaki S, Kawana S, Yasuoka S, Kawanami O.
|
| タイトル |
Human airway trypsin-like protease induces PAR-2-mediated IL-8 release in psoriasis vulgaris.
|
| ジャーナル |
J Invest Dermatol
|
| Abstract |
Human airway trypsin-like protease (HAT), a novel serine protease in the airways, enhances cell growth and IL-8 production. The expression and role of HAT in the skin however, is unknown. Immunofluorescence staining and reverse transcription (RT)-PCR were done to know HAT production in normal and psoriatic tissues and keratinocyte cell lines. Cell growth and/or IL-8 release analyses were made by bromo-deoxyuridine (BrdU) uptake and ELISA. Psoriatic epidermis showed more extensive immunofluorescence expression of HAT, and less extensive expression of protease-activated receptor (PAR)-2. RT-PCR demonstrated a higher HAT and a lesser PAR-2 mRNA expressions in psoriatic epidermis. Normal keratinocyte and epidermoid carcinoma cell lines expressed HAT and PAR-2 mRNA, and immortalized keratinocytes (HaCaT) expressed PAR-2, but not HAT mRNA. PAR-2 was detected along the keratinocyte surface in culture and became invisible upon HAT stimulation, suggesting a process of its internalization. HAT or PAR-2 activating peptide did not enhance BrdU uptake, but induced an IL-8 release. Treatment with HAT and IL-1beta synergistically increased the effect of IL-8 release. Inhibition of PAR-2 resulted in a decreased HAT-induced IL-8 release. Thus, HAT might promote PAR-2-mediated IL-8 production to accumulate inflammatory cells in the epidermal layer of psoriasis.
|
| 巻・号 |
122(4)
|
| ページ |
937-44
|
| 公開日 |
2004-4-1
|
| DOI |
10.1111/j.0022-202X.2004.22415.x
|
| PII |
S0022-202X(15)30765-X
|
| PMID |
15102084
|
| MeSH |
Adult
Aged
Biological Transport / drug effects
Biopsy
Cell Division / drug effects
Cells, Cultured
DNA / biosynthesis
Epidermis / metabolism
Epidermis / pathology
Female
Humans
Interleukin-18 / metabolism*
Keratinocytes / metabolism
Male
Middle Aged
Psoriasis / metabolism*
Psoriasis / pathology
RNA, Messenger / metabolism
Receptor, PAR-2 / genetics
Receptor, PAR-2 / metabolism*
Recombinant Proteins / pharmacology
Serine Endopeptidases / genetics
Serine Endopeptidases / metabolism*
Serine Endopeptidases / pharmacology
Skin / metabolism
Skin / pathology
Tissue Distribution
|
| IF |
7.143
|
| 引用数 |
44
|
|
WOS 分野
|
DERMATOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
A431(RCB0202) |
