RRC ID 42150
著者 Nakamori M, Fu X, Rousseau R, Chen SY, Zhang X.
タイトル Destruction of nonimmunogenic mammary tumor cells by a fusogenic oncolytic herpes simplex virus induces potent antitumor immunity.
ジャーナル Mol Ther
Abstract In principle, destruction of tumor cells in vivo by oncolytic agents would release the entire repertoire of tumor antigens in their natural forms, leading to effective antitumor immunity. This goal has been elusive despite extensive testing of numerous strategies. We developed a doubly fusogenic oncolytic herpes simplex virus (Synco-2D) that kills tumor cells by a unique dual mechanism combining direct cytolysis with syncytial formation induced by cell membrane fusion. A single intratumor injection of Synco-2D induced strong antitumor immunity against an otherwise nonimmunogenic murine mammary tumor growing in immune-competent mice. CD8+ T cells were the primary mediators of immunity, contributing to the destruction of both primary and metastatic tumors. We conclude that the fusogenic capacity of Synco-2D enables it to elicit antitumor immunity exceeding that induced by more conventional oncolytic viruses.
巻・号 9(5)
ページ 658-65
公開日 2004-5-1
DOI 10.1016/j.ymthe.2004.02.019
PII S1525-0016(04)00085-1
PMID 15120326
MeSH Animals Carcinoma / immunology Carcinoma / virology Cell Line Cell Survival Female Interferon-gamma / immunology Interleukin-10 / immunology Lung Neoplasms / pathology Lung Neoplasms / secondary Lymphocyte Depletion Mammary Neoplasms, Experimental / immunology* Mammary Neoplasms, Experimental / virology Membrane Fusion Mice Mice, Inbred BALB C Neoplasm Metastasis / pathology Neoplasm Transplantation Simplexvirus / physiology* T-Lymphocytes, Cytotoxic / immunology Tumor Escape / immunology
IF 8.986
引用数 47
WOS 分野 MEDICINE, RESEARCH & EXPERIMENTAL BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY
リソース情報
ヒト・動物細胞 Meth-A(RCB0464)