論文 - 詳細
| RRC ID | 42159 |
|---|---|
| 著者 | Misawa E, Sakurai T, Yamada M, Tamura Y, Motoyoshi K. |
| タイトル | Administration of macrophage colony-stimulating factor mobilized both CD11b+CD11c+ cells and NK1.1+ cells into peripheral blood. |
| ジャーナル | Int Immunopharmacol |
| Abstract |
We attempted the phenotypic characterization of peripheral blood (PB) cells after daily administration of macrophage colony-stimulating factor (M-CSF) in mice. The number of CD11b+ cells was increased by M-CSF treatment (2- and 5-day injections). Notably, CD11bbrightCD11cdim, CD11b+CD11c+ and CD11b+CD80+ cells were significantly increased by 2-day treatment of M-CSF. On the other hand, the number of NK1.1+ cells was not changed by the 2-day treatment, but it was significantly increased by the 5-day treatment. However, the numbers of CD3+ and NK1.1+CD3+ cells were not changed by M-CSF treatment. Then, mononuclear cells (MNCs) were separated from the PB of mice treated with saline or M-CSF, and they were incubated with GM-CSF + IL-4 or IL-2. Compared with the saline-treated one (S-MNCs), the MNCs of M-CSF-treated mice (M-MNCs) showed strong proliferation by the GM-CSF + IL-4 stimulation. The MNCs could stimulate proliferation of allo-T cells in the mixed lymphocyte reaction (MLR), especially the M-MNCs showed strong reaction. On the other hand, the stimulation by IL-2 induced strong cell growth of MNCs. And M-CSF treatment enhanced this response. Furthermore, the M-MNCs (stimulated by IL-2 in vitro) exhibited greater cytotoxicity against Yac-1 cells than the S-MNCs. In conclusion, we found that administration of M-CSF mobilized CD11b+, CD11b+CD11c+, CD11b+CD80+, and NK1.1+cells into PB. And the injection of M-CSF facilitates the generation of dendritic and natural killer cells from PB cells in vitro. These results suggest that the mobilized cells may provide for application of immunotherapy. |
| 巻・号 | 4(6) |
| ページ | 791-803 |
| 公開日 | 2004-6-1 |
| DOI | 10.1016/j.intimp.2004.03.004 |
| PII | S1567-5769(04)00081-5 |
| PMID | 15135320 |
| MeSH | Animals Antigens / metabolism* Antigens, Ly Antigens, Surface B7-1 Antigen / metabolism CD11b Antigen / metabolism* CD11c Antigen / metabolism* CD3 Complex / metabolism Colony-Stimulating Factors / pharmacology Cytokines / biosynthesis Dendritic Cells / drug effects Dendritic Cells / metabolism Killer Cells, Natural / drug effects Killer Cells, Natural / metabolism* Lectins, C-Type Lymphocyte Culture Test, Mixed Macrophage Colony-Stimulating Factor / pharmacology* Male Mice Mice, Inbred BALB C Mice, Inbred C57BL NK Cell Lectin-Like Receptor Subfamily B Phenotype Proteins / metabolism* Recombinant Proteins |
| IF | 3.943 |
| 引用数 | 6 |
| WOS 分野 | PHARMACOLOGY & PHARMACY IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | YAC-1(RCB1165) |