Reference - Detail
| RRC ID | 42163 |
|---|---|
| Author | Nakatsu Y, Yamada K, Ueda J, Onogi A, Ables GP, Nishibori M, Hata H, Takada A, Sawai K, Tanabe Y, Morita M, Daikohara M, Watanabe T. |
| Title | Genetic polymorphisms and antiviral activity in the bovine MX1 gene. |
| Journal | Anim Genet |
| Abstract |
Bovine MX1 cDNAs consisting of 2280 bp from 11 animals of five breeds and from a cultured cell line were sequenced and compared with previously reported data. Ten nucleotide substitutions were synonymous mutations, and a single nucleotide substitution at 458 resulted in an amino acid exchange of Ile (ATT) and Met (ATG). A 13-bp deletion-insertion mutation was also found in the 3'-UTR. Based on the nucleotide substitutions found in this study, bovine MX1 cDNA was classified into 11 genotypes. A phylogenetic tree of the 11 genotypes suggested that the genotypes observed in Brahman were a great genetic distance from other genotypes. An 18-bp deletion-insertion variation at position 171 was found to be the result of alternative splicing. The 18-bp deletion-insertion is located at the boundary between exon 3 and intron 3. Permanently transfected 3T3 cell lines expressing bovine MX1 mRNA were established to analyse the antiviral potential against VSVDeltaG*-G infection. Transfected cell clones expressing bovine MX1 mRNA showed a significantly smaller number of cells infected with VSVDeltaG*-G compared with the control cells. These results indicate that the bovine MX1 protein has potent antiviral activity. |
| Volume | 35(3) |
| Pages | 182-7 |
| Published | 2004-6-1 |
| DOI | 10.1111/j.1365-2052.2004.01125.x |
| PII | AGE1125 |
| PMID | 15147388 |
| MeSH | 3T3 Cells Alternative Splicing Animals Antiviral Agents / genetics* Base Sequence Cattle / genetics* Cluster Analysis DNA Primers DNA, Complementary / genetics GTP-Binding Proteins / classification GTP-Binding Proteins / genetics* Mice Molecular Sequence Data Mutation, Missense / genetics Myxovirus Resistance Proteins Phylogeny* Polymorphism, Genetic* Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA Transfection Vesicular stomatitis Indiana virus / genetics* |
| IF | 2.841 |
| Times Cited | 15 |
| WOS Category | AGRICULTURE, DAIRY & ANIMAL SCIENCE GENETICS & HEREDITY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 3T3 fibroblast cells from a BALB/c mouse |