Reference - Detail
|Author||Okumura S, Akao T, Higuchi K, Saitoh H, Mizuki E, Ohba M, Inouye K.|
|Title||Bacillus thuringiensis serovar shandongiensis strain 89-T-34-22 produces multiple cytotoxic proteins with similar molecular masses against human cancer cells.|
|Journal||Lett. Appl. Microbiol.|
AIMS:To prove that Bacillus thuringiensis serovar shandongiensis strain 89-T-34-22 produces several novel cytotoxic proteins against human leukaemic T cells.
METHODS AND RESULTS:Parasporal inclusion protein was solubilized and processed by proteinase K and was separated by anion-exchange chromatography. Cytopathic effects of each fraction against MOLT-4 and Jurkat cells were monitored.
CONCLUSIONS:Existence of at least two novel cytotoxic proteins was suggested and N-terminal sequences of the newly identified proteins were determined to be QSTTDVIREY and X (Y or I) (P or I) NLANELA (X indicates uncertain amino acids). Molecular masses of the two proteins were approx. 27-28 kDa.
SIGNIFICANCE AND IMPACT OF THE STUDY:In this study, we demonstrated that the strain 89-T-34-22 produces at least two novel cytotoxic proteins with similar molecular masses against human cancer cells. This is the first strain of B. thuringiensis which produces multiple cytotoxic proteins against human cancer cells.
|MeSH||Amino Acid Sequence Antineoplastic Agents / chemistry Antineoplastic Agents / metabolism Antineoplastic Agents / toxicity* Bacillus thuringiensis / metabolism* Bacterial Proteins / chemistry Bacterial Proteins / metabolism Bacterial Proteins / toxicity* Cytotoxins / chemistry Cytotoxins / metabolism Cytotoxins / toxicity* HeLa Cells / drug effects Humans Jurkat Cells Leukemia, T-Cell Tumor Cells, Cultured / drug effects|
|WOS Category||BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY|
|Human and Animal Cells|