論文 - 詳細
| RRC ID | 4219 |
|---|---|
| 著者 | Das PP, Bagijn MP, Goldstein LD, Woolford JR, Lehrbach NJ, Sapetschnig A, Buhecha HR, Gilchrist MJ, Howe KL, Stark R, Matthews N, Berezikov E, Ketting RF, Tavaré S, Miska EA. |
| タイトル | Piwi and piRNAs act upstream of an endogenous siRNA pathway to suppress Tc3 transposon mobility in the Caenorhabditis elegans germline. |
| ジャーナル | Mol Cell |
| Abstract |
The Piwi proteins of the Argonaute superfamily are required for normal germline development in Drosophila, zebrafish, and mice and associate with 24-30 nucleotide RNAs termed piRNAs. We identify a class of 21 nucleotide RNAs, previously named 21U-RNAs, as the piRNAs of C. elegans. Piwi and piRNA expression is restricted to the male and female germline and independent of many proteins in other small-RNA pathways, including DCR-1. We show that Piwi is specifically required to silence Tc3, but not other Tc/mariner DNA transposons. Tc3 excision rates in the germline are increased at least 100-fold in piwi mutants as compared to wild-type. We find no evidence for a Ping-Pong model for piRNA amplification in C. elegans. Instead, we demonstrate that Piwi acts upstream of an endogenous siRNA pathway in Tc3 silencing. These data might suggest a link between piRNA and siRNA function. |
| 巻・号 | 31(1) |
| ページ | 79-90 |
| 公開日 | 2008-7-11 |
| DOI | 10.1016/j.molcel.2008.06.003 |
| PII | S1097-2765(08)00392-4 |
| PMID | 18571451 |
| PMC | PMC3353317 |
| MeSH | Animals Argonaute Proteins Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* DNA Transposable Elements / genetics* Drosophila Proteins Female Gene Silencing Genes, Helminth Germ Cells / growth & development Germ Cells / metabolism* Male Proteins / genetics Proteins / metabolism* RNA, Helminth / metabolism RNA, Small Interfering / metabolism* RNA-Induced Silencing Complex Transposases / metabolism |
| IF | 15.584 |
| 引用数 | 226 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
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| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 25 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm872 |