| RRC ID |
42239
|
| Author |
Boros S, Kamps B, Wunderink L, de Bruijn W, de Jong WW, Boelens WC.
|
| Title |
Transglutaminase catalyzes differential crosslinking of small heat shock proteins and amyloid-beta.
|
| Journal |
FEBS Lett
|
| Abstract |
Crosslinking of proteins by tissue transglutaminase (tTG) is enhanced in amyloid (Abeta) deposits characteristic of Alzheimer's disease and sporadic inclusion body myositis. Small heat shock proteins (sHsps) also occur in amyloid deposits. We here report the substrate characteristics for tTG of six sHsps. Hsp27, Hsp20 and HspB8 are both lysine- and glutamine-donors, alphaB-crystallin only is a lysine-donor, HspB2 a glutamine-donor, and HspB3 no substrate at all. Close interaction of proteins stimulates crosslinking efficiency as crosslinking between different sHsps only takes place within the same heteromeric complex. We also observed that alphaB-crystallin, Hsp27 and Hsp20 associate with Abeta in vitro, and can be readily crosslinked by tTG.
|
| Volume |
576(1-2)
|
| Pages |
57-62
|
| Published |
2004-10-8
|
| DOI |
10.1016/j.febslet.2004.08.062
|
| PII |
S0014579304010816
|
| PMID |
15474010
|
| MeSH |
Amyloid beta-Peptides / metabolism*
Blotting, Western
Catalysis
Cross-Linking Reagents / metabolism*
Electrophoresis, Polyacrylamide Gel
Heat-Shock Proteins / genetics
Heat-Shock Proteins / metabolism*
Humans
Recombinant Proteins / metabolism
Transglutaminases / metabolism*
|
| IF |
3.057
|
| Times Cited |
32
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
anti-αB-crystallin(RCB1304) |
