Reference - Detail
| RRC ID | 4223 |
|---|---|
| Author | Doitsidou M, Flames N, Lee AC, Boyanov A, Hobert O. |
| Title | Automated screening for mutants affecting dopaminergic-neuron specification in C. elegans. |
| Journal | Nat Methods |
| Abstract |
We describe an automated method to isolate mutant Caenorhabditis elegans that do not appropriately execute cellular differentiation programs. We used a fluorescence-activated sorting mechanism implemented in the COPAS Biosort machine to isolate mutants with subtle alterations in the cellular specificity of GFP expression. This methodology is considerably more efficient than comparable manual screens and enabled us to isolate mutants in which dopamine neurons do not differentiate appropriately. |
| Volume | 5(10) |
| Pages | 869-72 |
| Published | 2008-10-1 |
| DOI | 10.1038/nmeth.1250 |
| PII | nmeth.1250 |
| PMID | 18758453 |
| PMC | PMC2693092 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Dopamine / metabolism* Flow Cytometry Fluorescence Green Fluorescent Proteins / genetics Mutation* Neurons / metabolism* |
| IF | 30.822 |
| Times Cited | 55 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS |
| Resource | |
| C.elegans | tm2044 tm1446 |