Reference - Detail
| RRC ID | 42242 |
|---|---|
| Author | Konno T, Hasuda H, Ishihara K, Ito Y. |
| Title | Photo-immobilization of a phospholipid polymer for surface modification. |
| Journal | Biomaterials |
| Abstract |
A photo-reactive polymer having a phospholipid polar group was prepared, and the polymer was photo-immobilized on polymeric surfaces, where its interactions with biocomponents were investigated. By using a photo-immobilization method, the polymer was used for surface modification of polyethylene and polypropylene, polymers whose surfaces were not treated in our previous development of the phosphorylcholine-derived polymer. The photo-reactive polymer was synthesized by a coupling reaction involving copolymer consisting of 2-methacryloyloxyethyl phosphorylcholine and methacrylic acid with 4-azidoaniline. When the polymer was unpattern immobilized on the surface, X-ray photo-electron spectroscopic analysis and static contact angle measurements were performed. It was shown that the surface was covered with phospholipid polar groups. Micropattern immobilization was carried out using a micropatterned photo-mask. Measurements using atomic force microscopy showed that the swelled micropatterned polymer was five times as thick as the dried one. Protein adsorption and platelet adhesion were reduced on the polymer-immobilized regions. Mammalian cells did not adhere, and formed aggregates on the immobilized regions. In conclusion, the photo-reactive phospholipid polymer was covalently immobilized on the conventional polymer surfaces and it tended to reduce interactions with proteins and cells. |
| Volume | 26(12) |
| Pages | 1381-8 |
| Published | 2005-4-1 |
| DOI | 10.1016/j.biomaterials.2004.04.047 |
| PII | S0142961204004466 |
| PMID | 15482825 |
| MeSH | Adsorption / radiation effects Animals Cell Adhesion / physiology* Cells, Cultured Coated Materials, Biocompatible / chemistry Humans Immunoglobulins / chemistry Light Materials Testing Mice Monocytes / cytology Monocytes / physiology Phospholipids / chemistry* Phospholipids / radiation effects Photochemistry / methods* Platelet Adhesiveness / physiology* Polyethylene / chemistry* Polyethylene / radiation effects Polypropylenes / chemistry* Polypropylenes / radiation effects Protein Binding Serum Albumin, Bovine / chemistry Surface Properties Tissue Engineering / methods |
| IF | 10.317 |
| Times Cited | 60 |
| WOS Category | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 11 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |