RRC ID 4226
Author Fischer SE, Butler MD, Pan Q, Ruvkun G.
Title Trans-splicing in C. elegans generates the negative RNAi regulator ERI-6/7.
Journal Nature
Abstract Mutations that enhance the response to double-stranded RNA (dsRNA) have revealed components of the RNA interference (RNAi) pathway or related small RNA pathways. To explore these small RNA pathways, we screened for Caenorhabditis elegans mutants displaying an enhanced response to exogenous dsRNAs. Here we describe the isolation of mutations in two adjacent, divergently transcribed open reading frames (eri-6 and eri-7) that fail to complement. eri-6 and eri-7 produce separate pre-messenger RNAs (pre-mRNAs) that are trans-spliced to form a functional mRNA, eri-6/7. Trans-splicing of eri-6/7 is mediated by a direct repeat that flanks the eri-6 gene. Adenosine to inosine editing within untranslated regions of eri-6 and eri-7 pre-mRNAs reveals a double-stranded pre-mRNA intermediate, forming in the nucleus before splicing occurs. The ERI-6/7 protein is a superfamily I helicase that both negatively regulates the exogenous RNAi pathway and functions in an endogenous RNAi pathway.
Volume 455(7212)
Pages 491-6
Published 2008-9-25
DOI 10.1038/nature07274
PII nature07274
PMID 18784652
PMC PMC2756026
MeSH Animals Caenorhabditis elegans / classification Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* DNA Helicases / genetics* DNA Helicases / metabolism* Genes, Helminth / genetics* Genetic Complementation Test Molecular Sequence Data Mutation / genetics Open Reading Frames / genetics RNA Interference / physiology* Repetitive Sequences, Nucleic Acid / genetics Trans-Splicing*
IF 41.577
Times Cited 48
C.elegans tm1887 tm1716 tm1917