| RRC ID |
42265
|
| 著者 |
Konishi J, Yamazaki K, Yokouchi H, Shinagawa N, Iwabuchi K, Nishimura M.
|
| タイトル |
The characteristics of human NKT cells in lung cancer--CD1d independent cytotoxicity against lung cancer cells by NKT cells and decreased human NKT cell response in lung cancer patients.
|
| ジャーナル |
Hum Immunol
|
| Abstract |
The activation of human Valpha24+Vbeta11+natural killer T cells (NKT) cells (Valpha24 NKT cells) induces effective antitumor responses with secondary immune effects through activation of conventional T cells and natural killer cells. In this study, we attempted to analyze the characteristics of human NKT cells in lung cancer patients. Valpha24 NKT cells stimulated with alpha-GalCer from healthy volunteers exhibited direct cytotoxic activity against two (RERF-LC-OK and PC-3) of seven human lung cancer cell lines studied. Cytotoxicity by Valpha24 NKT cells against human lung cancer cells was dependent on the perforin pathway and independent of Fas/FasL pathway. Intracellular adhesion molecule (ICAM)-1 expression on tumor cells was clearly associated with the cytotoxicity of Valpha24 NKT cells. On the other hand, the proportion of Valpha24 NKT cells in the patients with lung cancer was lower than that in the healthy volunteers. Furthermore, the proliferative response of Valpha24 NKT cells to alpha-GalCer was significantly lower in the peripheral blood mononuclear cells in the patients with lung cancer. Addition of granulocyte colony-stimulating factor moderately restored the low proliferative response of Valpha24 NKT cells in the patients with lung cancer, however the percentage by which the response was restored in these patients was still lower than the natural response in healthy volunteers. These results suggest that Valpha24 NKT cells may play a pivotal role or the antitumor response in lung cancer.
|
| 巻・号 |
65(11)
|
| ページ |
1377-88
|
| 公開日 |
2004-11-1
|
| DOI |
10.1016/j.humimm.2004.09.003
|
| PII |
S0198-8859(04)00623-8
|
| PMID |
15556688
|
| MeSH |
Adult
Aged
Aged, 80 and over
Antibodies, Monoclonal / pharmacology
Antigens, CD / analysis
Antigens, CD1 / immunology*
Antigens, CD1d
CD4-Positive T-Lymphocytes / chemistry
CD4-Positive T-Lymphocytes / immunology
CD4-Positive T-Lymphocytes / metabolism
CD8-Positive T-Lymphocytes / chemistry
CD8-Positive T-Lymphocytes / immunology
CD8-Positive T-Lymphocytes / metabolism
Cell Line, Tumor
Cell Proliferation / drug effects
Cytokines / metabolism
Cytotoxicity Tests, Immunologic
Cytotoxicity, Immunologic / immunology*
Female
Galactosylceramides / pharmacology
Humans
Killer Cells, Natural / chemistry
Killer Cells, Natural / immunology*
Killer Cells, Natural / metabolism
Leukocytes, Mononuclear / drug effects
Leukocytes, Mononuclear / immunology
Leukocytes, Mononuclear / metabolism
Lung Neoplasms / immunology*
Lymphocyte Activation / drug effects
Lymphocyte Activation / immunology
Lymphocyte Subsets / chemistry
Lymphocyte Subsets / immunology*
Lymphocyte Subsets / metabolism
Male
Membrane Glycoproteins / antagonists & inhibitors
Membrane Glycoproteins / metabolism
Middle Aged
Perforin
Pore Forming Cytotoxic Proteins
Receptors, Antigen, T-Cell, alpha-beta / analysis
|
| IF |
2.412
|
| 引用数 |
29
|
|
WOS 分野
|
IMMUNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
PC-3(RCB2145)
A549(RCB0098)
LK-2(RCB1970)
EBC-1(RCB1965)
MS-1(RCB0725)
Lu-134-A(RCB0466) |
