| RRC ID |
42272
|
| 著者 |
Guillemette J, Caron AZ, Regimbald-Dumas Y, Arguin G, Mignery GA, Boulay G, Guillemette G.
|
| タイトル |
Expression of a truncated form of inositol 1,4,5-trisphosphate receptor type III in the cytosol of DT40 triple inositol 1,4,5-trisphosphate receptor-knockout cells.
|
| ジャーナル |
Cell Calcium
|
| Abstract |
In non-excitable cells, the inositol 1,4,5-trisphosphate receptor (IP3R) is an intracellular Ca2+ channel playing a major role in Ca2+ signaling. Three isoforms of IP3R have been identified and most cell types express different proportions of each isoform. The DT40 B lymphocyte cell line lacking all three IP3R isoforms (DT40IP3R-KO cells) represents an excellent model to re-express any recombinant IP3R and analyze its specific properties. In the study presented here, we confirmed that DT40IP3R-KO cells do not express any IP3-sensitive Ca2+ release channel. However, with an immunoblot approach and a [3H]IP3 binding approach we demonstrated the presence of a C-terminally truncated form of IP3R type III in the cytosolic fraction of DT40IP3R-KO cells. We further showed that this truncated IP3R retained the ability to couple to the Ca2+ entry channel TRPC6. Therefore, a word of caution is offered about the interpretation of results obtained in using DT40IP3R-KO cells to study the cellular mechanisms of Ca2+ entry.
|
| 巻・号 |
37(2)
|
| ページ |
97-104
|
| 公開日 |
2005-2-1
|
| DOI |
10.1016/j.ceca.2004.03.005
|
| PII |
S0143416004000867
|
| PMID |
15589990
|
| MeSH |
Animals
Calcium / metabolism
Calcium Channels / biosynthesis
Calcium Channels / genetics*
Calcium Channels / immunology
Cells, Cultured
Chickens / genetics
Chickens / metabolism
Immunoblotting
Inositol 1,4,5-Trisphosphate / metabolism*
Inositol 1,4,5-Trisphosphate Receptors
Ion Channels / metabolism
Receptors, Cytoplasmic and Nuclear / biosynthesis
Receptors, Cytoplasmic and Nuclear / genetics*
Receptors, Cytoplasmic and Nuclear / immunology
TRPC Cation Channels
Tritium / metabolism
|
| IF |
4.874
|
| 引用数 |
8
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
IP3R^(-) DT40(RCB1467) |
