| RRC ID |
42306
|
| Author |
Yamashita Y, Shimada M, Harimoto N, Tanaka S, Shirabe K, Ijima H, Nakazawa K, Fukuda J, Funatsu K, Maehara Y.
|
| Title |
cDNA microarray analysis in hepatocyte differentiation in Huh 7 cells.
|
| Journal |
Cell Transplant
|
| Abstract |
The risk of xenozoonosis infections poses the greatest obstacle against the clinical application of a hybrid artificial liver support system (HALSS). Primary human hepatocytes are an ideal source for HALSS, but the shortage of human livers available for hepatocyte isolation limits this modality. To resolve this issue, we previously demonstrated the upregulation of hepatocyte-specific function by spheroid formation in polyurethane foam and by culturing with the histone deacetylase inhibitor, trichostatin A (TSA), in a human hepatoma cell line (Huh 7). In this article we analyze the gene expression profile using cDNA microarray (1281 genes) in spheroid formation or culturing with TSA in Huh 7 to determine the target genes in hepatocyte differentiation. In both the spheroid formation and in the culture with TSA, the Oct-3/4 transcription factor was upregulated more thantwofold, while the early growth response-1 (EGR-1) transactivator was downregulated less than 0.5-fold. These results indicate that expressions of Oct-3/4 and EGR-1 may be key factors in the induction of hepatocyte differentiation in Huh 7.
|
| Volume |
13(7-8)
|
| Pages |
793-9
|
| Published |
2004-1-1
|
| DOI |
10.3727/000000004783983396
|
| PMID |
15690981
|
| MeSH |
Cell Culture Techniques / methods
Cell Culture Techniques / trends
Cell Differentiation / genetics*
Cell Line, Tumor
Cell Proliferation
DNA, Complementary / analysis*
DNA, Complementary / genetics
DNA-Binding Proteins / genetics
Down-Regulation / genetics
Early Growth Response Protein 1
Enzyme Inhibitors / pharmacology
Gene Expression Profiling* / methods
Gene Expression Regulation / genetics
Hepatocytes / cytology*
Hepatocytes / physiology*
Humans
Hydroxamic Acids / pharmacology
Immediate-Early Proteins / genetics
Liver, Artificial / trends
Octamer Transcription Factor-3
Oligonucleotide Array Sequence Analysis*
Reverse Transcriptase Polymerase Chain Reaction
Spheroids, Cellular / cytology
Spheroids, Cellular / physiology
Transcription Factors / genetics
Up-Regulation / genetics
|
| IF |
3.341
|
| Times Cited |
19
|
| Resource |
| Human and Animal Cells |
HuH-7(RCB1366) |
