RRC ID 42343
著者 Xia HH, Lam SK, Chan AO, Lin MC, Kung HF, Ogura K, Berg DE, Wong BC.
タイトル Macrophage migration inhibitory factor stimulated by Helicobacter pylori increases proliferation of gastric epithelial cells.
ジャーナル World J Gastroenterol
Abstract AIM:Helicobacter pylori (H pylori) is associated with increased gastric inflammatory and epithelial expression of macrophage migration inhibitory factor (MIF) and gastric epithelial cell proliferation. This study aimed at determining whether H pylori directly stimulates release of MIF in monocytes, whether the cag pathogenicity island (PAI) is involved for this function, and whether MIF stimulated by H pylori increases gastric epithelial cell proliferation in vitro.
METHODS:A cytotoxic wild-type H pylori strain (TN2), its three isogenic mutants (TN2Deltacag, TN2DeltacagA and TN2DeltacagE) were co-cultured with cells of a human monocyte cell line, THP-1, for 24 h at different organism/cell ratios. MIF in the supernatants was measured by an ELISA. Cells of a human gastric cancer cell line, MKN45, were then co-cultured with the supernatants, with and without monoclonal anti-MIF antibody for 24 h. The cells were further incubated for 12 h after addition of 3H-thymidine, and the levels of incorporation of 3H-thymidine were measured with a liquid scintillation counter.
RESULTS:The wild-type strain and the isogenic mutants, TN2DeltacagA and TN2 DeltacagE, increased MIF release at organism/cell ratios of 200/1 and 400/1, but not at the ratios of 50/1 and 100/1. However, the mutant TN2delta cag did not increase the release of MIF at any of the four ratios. 3H-thymidine readings for MKN-45 cells were significantly increased with supernatants derived from the wild-type strain and the mutants TN2DeltacagA and TN2DeltacagE, but not from the mutant TN2Deltacag. Moreover, in the presence of monoclonal anti-MIF antibody, the stimulatory effects of the wild-type strain on cell proliferation disappeared.
CONCLUSION:H pylori stimulates MIF release in monocytes, likely through its cag PAI, but not related to cagA or cagE. H pylori-stimulated monocyte culture supernatant increases gastric cell proliferation, which is blocked by anti-MIF antibody, suggesting that MIF plays an important role in H pylori-induced gastric epithelial cell proliferation.
巻・号 11(13)
ページ 1946-50
公開日 2005-4-7
DOI 10.3748/wjg.v11.i13.1946
PMID 15800984
PMC PMC4305715
MeSH Antibodies, Monoclonal / pharmacology Antigens, Bacterial / genetics Bacterial Proteins / genetics Cell Division / physiology Cell Line Epithelial Cells / metabolism Epithelial Cells / microbiology Epithelial Cells / pathology Helicobacter Infections / pathology* Helicobacter pylori* / genetics Humans In Vitro Techniques Macrophage Migration-Inhibitory Factors / immunology Macrophage Migration-Inhibitory Factors / metabolism* Monocytes / cytology Monocytes / microbiology* Mutation Stomach / microbiology* Stomach / pathology
IF 3.665
引用数 21
WOS 分野 GASTROENTEROLOGY & HEPATOLOGY
リソース情報
ヒト・動物細胞 MKN45(RCB1001)