論文 - 詳細
RRC ID | 42343 |
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著者 | Xia HH, Lam SK, Chan AO, Lin MC, Kung HF, Ogura K, Berg DE, Wong BC. |
タイトル | Macrophage migration inhibitory factor stimulated by Helicobacter pylori increases proliferation of gastric epithelial cells. |
ジャーナル | World J Gastroenterol |
Abstract |
AIM:Helicobacter pylori (H pylori) is associated with increased gastric inflammatory and epithelial expression of macrophage migration inhibitory factor (MIF) and gastric epithelial cell proliferation. This study aimed at determining whether H pylori directly stimulates release of MIF in monocytes, whether the cag pathogenicity island (PAI) is involved for this function, and whether MIF stimulated by H pylori increases gastric epithelial cell proliferation in vitro. METHODS:A cytotoxic wild-type H pylori strain (TN2), its three isogenic mutants (TN2Deltacag, TN2DeltacagA and TN2DeltacagE) were co-cultured with cells of a human monocyte cell line, THP-1, for 24 h at different organism/cell ratios. MIF in the supernatants was measured by an ELISA. Cells of a human gastric cancer cell line, MKN45, were then co-cultured with the supernatants, with and without monoclonal anti-MIF antibody for 24 h. The cells were further incubated for 12 h after addition of 3H-thymidine, and the levels of incorporation of 3H-thymidine were measured with a liquid scintillation counter. RESULTS:The wild-type strain and the isogenic mutants, TN2DeltacagA and TN2 DeltacagE, increased MIF release at organism/cell ratios of 200/1 and 400/1, but not at the ratios of 50/1 and 100/1. However, the mutant TN2delta cag did not increase the release of MIF at any of the four ratios. 3H-thymidine readings for MKN-45 cells were significantly increased with supernatants derived from the wild-type strain and the mutants TN2DeltacagA and TN2DeltacagE, but not from the mutant TN2Deltacag. Moreover, in the presence of monoclonal anti-MIF antibody, the stimulatory effects of the wild-type strain on cell proliferation disappeared. CONCLUSION:H pylori stimulates MIF release in monocytes, likely through its cag PAI, but not related to cagA or cagE. H pylori-stimulated monocyte culture supernatant increases gastric cell proliferation, which is blocked by anti-MIF antibody, suggesting that MIF plays an important role in H pylori-induced gastric epithelial cell proliferation. |
巻・号 | 11(13) |
ページ | 1946-50 |
公開日 | 2005-4-7 |
DOI | 10.3748/wjg.v11.i13.1946 |
PMID | 15800984 |
PMC | PMC4305715 |
MeSH | Antibodies, Monoclonal / pharmacology Antigens, Bacterial / genetics Bacterial Proteins / genetics Cell Division / physiology Cell Line Epithelial Cells / metabolism Epithelial Cells / microbiology Epithelial Cells / pathology Helicobacter Infections / pathology* Helicobacter pylori* / genetics Humans In Vitro Techniques Macrophage Migration-Inhibitory Factors / immunology Macrophage Migration-Inhibitory Factors / metabolism* Monocytes / cytology Monocytes / microbiology* Mutation Stomach / microbiology* Stomach / pathology |
IF | 3.665 |
引用数 | 21 |
WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY |
リソース情報 | |
ヒト・動物細胞 | MKN45(RCB1001) |