論文 - 詳細
| RRC ID | 4235 |
|---|---|
| 著者 | Grigsby IF, Finger FP. |
| タイトル | UNC-85, a C. elegans homolog of the histone chaperone Asf1, functions in post-embryonic neuroblast replication. |
| ジャーナル | Dev Biol |
| Abstract |
Normal animal development requires accurate cell divisions, not only in the early stages of rapid embryonic cleavages, but also in later developmental stages. The Caenorhabditis elegans unc-85 gene is implicated only in cell divisions that occur post-embryonically, primarily in terminal neuronal lineages. Variable post-embryonic cell division failures in ventral cord motoneuron precursors result in uncoordinated locomotion of unc-85 mutant larvae by the second larval stage. These neuroblast cell division failures often result in unequally sized daughter nuclei, and sometimes in nuclear fusions. Using a combination of conventional mapping techniques and microarray analysis, we cloned the unc-85 gene, and find that it encodes one of two C. elegans homologs of the yeast Anti-silencing function 1 (Asf1) histone chaperone. The unc-85 gene is expressed in replicating cells throughout development, and the protein is localized in nuclei. Examination of null mutants confirms that embryonic neuroblast cell divisions occur normally, but post-embryonic neuroblast cell divisions fail. Analysis of the DNA content of the mutant neurons indicates that defective replication in post-embryonic neuroblasts gives rise to ventral cord neurons with an average DNA content of approximately 2.5 n. We conclude that UNC-85 functions in post-embryonic DNA replication in ventral cord motor neuron precursors. |
| 巻・号 | 319(1) |
| ページ | 100-9 |
| 公開日 | 2008-7-1 |
| DOI | 10.1016/j.ydbio.2008.04.013 |
| PII | S0012-1606(08)00282-0 |
| PMID | 18490010 |
| MeSH | Amino Acid Sequence Animals Caenorhabditis elegans / embryology Caenorhabditis elegans / growth & development* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* Cell Nucleus / metabolism Cloning, Molecular DNA Replication Embryo, Nonmammalian / metabolism Gene Expression Regulation, Developmental Molecular Chaperones / genetics* Molecular Chaperones / metabolism* Molecular Sequence Data Motor Neurons / metabolism Neuroepithelial Cells Sequence Alignment |
| IF | 2.896 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm2812 |