RRC ID 42363
著者 Sakuma-Zenke M, Sakai A, Nakayamada S, Kunugita N, Tabata T, Uchida S, Tanaka S, Mori T, Nakai K, Tanaka Y, Nakamura T.
タイトル Reduced expression of platelet endothelial cell adhesion molecule-1 in bone marrow cells in mice after skeletal unloading.
ジャーナル J Bone Miner Res
Abstract UNLABELLED:One week of tail suspension significantly decreased the expression of PECAM-1 in mouse tibial bone marrow cells but not those of a number of other vascular factors. Anti-PECAM-1 antibody suppressed both ALP+ CFU-f formation and ALP production under co-culture of the osteoblastic cell line and the PECAM-1+ endothelial cell line. This study suggests that the reduced ALP activity after skeletal unloading is related to downregulation of PECAM-1 expression in bone marrow cells in mice.
INTRODUCTION:Vascular factors play a role in bone development and regeneration. We tested the hypothesis that skeletal unloading reduces osteogenic potential by inhibiting the molecules related to angiogenesis and/or vasculogenesis in bone marrow cells.
MATERIALS AND METHODS:Eight-week-old male mice were assigned to three groups after acclimatization for 1 week: ground control (GC), tail suspension (TS), and reloading after 7-day TS (RL). Bilateral tibial and humeral samples were used for analyses. MC3T3-E1, a mouse osteoblastic cell line, and EOMA and ISOS-1, mouse endothelial cell lines, were also used.
RESULTS:Flow cytometric analysis revealed that 7-day TS significantly decreased the expression of platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) in tibial bone marrow cells, but not those of angiopoietin-1, angiopoietin-2, Flk-1 (vascular endothelial growth factor receptor-2), and vascular endothelial cadherin. The expression of PECAM-1 in tibial marrow cells was reduced at day 3 of TS to 80% and still showed significantly low levels at day 7 of TS to 72% of that at the respective days of GC. This decreased expression of PECAM-1 after 7-day TS showed the GC level at 5-day reloading after 7-day TS. However, the expression of PECAM-1 in humeral marrow cells (internal bone marrow control) after TS and RL remained unchanged and equivalent to that of GC. The expression level of PECAM-1 mRNA was significantly lower at day 7 of TS to 62% of that in GC. Double labeling analyses revealed that PECAM-1+ cells mostly consisted of endothelial cells and partially of granulocytes. In bone marrow cell cultures, the formation of alkaline phosphatase (ALP)+ colony forming units-fibroblastic was significantly reduced in the presence of anti-PECAM-1 antibody in the medium compared with the presence of immunoglobulin G (0.025 times as much as ALP production with immunoglobulin G). ALP production by cultured MC3T3-E1 was enhanced in combination with PECAM-1+ EOMA (1.8 times as much as ALP production by MC3T3-E1 alone), but not in combination with PECAM-1- ISOS-1. Anti-PECAM-1 antibody inhibited the increase in ALP production under co-culture with EOMA.
CONCLUSIONS:Our data show that the reduced ALP activity after skeletal unloading is closely correlated with reduced expression of PECAM-1 in bone marrow cells. We speculate that the loss of osteogenic potential after skeletal unloading is caused by the suppression of PECAM-1 signaling on endothelial cellular surface.
巻・号 20(6)
ページ 1002-10
公開日 2005-6-1
DOI 10.1359/JBMR.050102
PMID 15883641
MeSH 3T3 Cells Alkaline Phosphatase / metabolism Angiopoietin-1 / biosynthesis Angiopoietin-2 / biosynthesis Animals Blood Platelets / metabolism* Body Weight Bone Development Bone Marrow Cells / cytology* Coculture Techniques Down-Regulation Endothelial Cells / cytology Endothelium, Vascular / metabolism Flow Cytometry Male Mice Mice, Inbred C57BL Neovascularization, Physiologic Osteoblasts / metabolism Platelet Endothelial Cell Adhesion Molecule-1 / biosynthesis* Reverse Transcriptase Polymerase Chain Reaction Stem Cells Temperature Tibia / cytology Tibia / metabolism Time Factors Vascular Endothelial Growth Factor Receptor-2 / biosynthesis
IF 5.854
引用数 8
WOS 分野 ENDOCRINOLOGY & METABOLISM
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)