RRC ID 42373
Author Cheng CM, Tsuneyama K, Matsui K, Takahashi H, Ishizawa S, Takano Y.
Title Cytoplasmic expression of c-erbB2 in non-small cell lung cancers.
Journal Virchows Arch.
Abstract The aim of this study was to investigate the expression of c-erbB2 in non-small cell lung cancers (NSCLCs) with attention both to membranous and cytoplasmic reaction, and to try to elucidate the meaning of cytoplasmic expression of c-erbB2 in NSCLCs. Immunohistochemical c-erbB2 expression and related clinico-pathological features were examined in 312 surgically resected patient tissues of NSCLCs, including 175 cases of adenocarcinoma and 137 cases of squamous cell carcinoma. Immunostaining of inner- and ecto-domain of c-erbB2, mRNA expression and the quantitation of soluble c-erbB2 in cultured media were performed in five NSCLC cell lines. Cytoplasmic expression of c-erbB2 was observed more frequently than membranous, both in patient tissues and cell lines. Neither membranous nor cytoplasmic expression of c-erbB2 was significantly correlated with short outcome in NSCLCs. Membranous c-erbB2 was expressed by both inner and ecto-domain, while cytoplasmic c-erbB2 was expressed by either or both inner and ecto-domain. c-erbB2 mRNA was produced in most cell lines; however, the soluble form was only detectable in a cell line that only presented a membranous c-erbB2. In conclusion, cytoplasmic c-erbB2 of NSCLCs was not a full-length protein only expressed in cellular membrane, but reflected degenerated c-erbB2 fragments with less functional ability.
Volume 446(6)
Pages 596-603
Published 2005-6
DOI 10.1007/s00428-005-1258-7
PMID 15902485
MeSH Adult Aged Aged, 80 and over Carcinoma, Non-Small-Cell Lung / metabolism* Carcinoma, Non-Small-Cell Lung / mortality Cell Line, Tumor Cell Membrane / metabolism* Cytoplasm / metabolism* Female Humans Immunoenzyme Techniques Immunohistochemistry Lung Neoplasms / metabolism* Lung Neoplasms / mortality Male Middle Aged Receptor, ErbB-2 / biosynthesis* Survival Analysis
IF 2.936
Times Cited 9
Human and Animal Cells