論文 - 詳細
| RRC ID | 42373 |
|---|---|
| 著者 | Cheng CM, Tsuneyama K, Matsui K, Takahashi H, Ishizawa S, Takano Y. |
| タイトル | Cytoplasmic expression of c-erbB2 in non-small cell lung cancers. |
| ジャーナル | Virchows Arch |
| Abstract |
The aim of this study was to investigate the expression of c-erbB2 in non-small cell lung cancers (NSCLCs) with attention both to membranous and cytoplasmic reaction, and to try to elucidate the meaning of cytoplasmic expression of c-erbB2 in NSCLCs. Immunohistochemical c-erbB2 expression and related clinico-pathological features were examined in 312 surgically resected patient tissues of NSCLCs, including 175 cases of adenocarcinoma and 137 cases of squamous cell carcinoma. Immunostaining of inner- and ecto-domain of c-erbB2, mRNA expression and the quantitation of soluble c-erbB2 in cultured media were performed in five NSCLC cell lines. Cytoplasmic expression of c-erbB2 was observed more frequently than membranous, both in patient tissues and cell lines. Neither membranous nor cytoplasmic expression of c-erbB2 was significantly correlated with short outcome in NSCLCs. Membranous c-erbB2 was expressed by both inner and ecto-domain, while cytoplasmic c-erbB2 was expressed by either or both inner and ecto-domain. c-erbB2 mRNA was produced in most cell lines; however, the soluble form was only detectable in a cell line that only presented a membranous c-erbB2. In conclusion, cytoplasmic c-erbB2 of NSCLCs was not a full-length protein only expressed in cellular membrane, but reflected degenerated c-erbB2 fragments with less functional ability. |
| 巻・号 | 446(6) |
| ページ | 596-603 |
| 公開日 | 2005-6-1 |
| DOI | 10.1007/s00428-005-1258-7 |
| PMID | 15902485 |
| MeSH | Adult Aged Aged, 80 and over Carcinoma, Non-Small-Cell Lung / metabolism* Carcinoma, Non-Small-Cell Lung / mortality Cell Line, Tumor Cell Membrane / metabolism* Cytoplasm / metabolism* Female Humans Immunoenzyme Techniques Immunohistochemistry Lung Neoplasms / metabolism* Lung Neoplasms / mortality Male Middle Aged Receptor, ErbB-2 / biosynthesis* Survival Analysis |
| IF | 2.906 |
| 引用数 | 10 |
| WOS 分野 | PATHOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | LC-1/sq(RCB0455) LC-1F(RCB0439) |