| RRC ID |
42375
|
| 著者 |
Hamasu T, Inanami O, Tsujitani M, Yokoyama K, Takahashi E, Kashiwakura I, Kuwabara M.
|
| タイトル |
Post-irradiation hypoxic incubation of X-irradiated MOLT-4 cells reduces apoptotic cell death by changing the intracellular redox state and modulating SAPK/JNK pathways.
|
| ジャーナル |
Apoptosis
|
| Abstract |
To elucidate radiobiological effects of hypoxia on X-ray-induced apoptosis, MOLT-4 cells were treated under four set of conditions: (1) both X irradiation and incubation under normoxia, (2) X irradiation under hypoxia and subsequent incubation under normoxia, (3) X irradiation under normoxia and subsequent incubation under hypoxia, and (4) both X irradiation and incubation under hypoxia, and the induction of apoptosis was examined by fluorescence microscopy. About 28-33% apoptosis was observed in cells treated under conditions 1 and 2, but this value was significantly reduced to around 18-20% in cells treated under conditions 3 and 4, suggesting that post-irradiation hypoxic incubation rather than hypoxic irradiation mainly caused the reduction of apoptosis. The activation and expression of apoptosis signal-related molecules SAPK/JNK, Fas and caspase-3 were also suppressed by hypoxic incubation. Effects of hypoxic incubation were canceled when cells were treated under conditions 3 and 4 with an oxygen-mimicking hypoxic cell radiosensitizer, whereas the addition of N-acetyl-L-cysteine again reduced the induction of apoptosis. From these results it was concluded that hypoxia reduced the induction of apoptosis by changing the intracellular redox state, followed by the regulation of apoptotic signals in X-irradiated MOLT-4 cells.
|
| 巻・号 |
10(3)
|
| ページ |
557-67
|
| 公開日 |
2005-5-1
|
| DOI |
10.1007/s10495-005-1888-x
|
| PMID |
15909118
|
| MeSH |
Acetylcysteine / pharmacology
Apoptosis / drug effects
Apoptosis / physiology*
Apoptosis / radiation effects*
Caspase 3
Caspases / metabolism
Cell Line, Tumor
Enzyme Activation
Glutathione / metabolism
Humans
Hypoxia / physiopathology*
Imidazoles / pharmacology
Leukemia
Microscopy, Fluorescence
Mitogen-Activated Protein Kinases / metabolism
Radiation-Sensitizing Agents / pharmacology
fas Receptor / biosynthesis
|
| IF |
4.543
|
| 引用数 |
12
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MOLT-4(RCB0206)
Jurkat(RCB0806) |
