| RRC ID |
42386
|
| Author |
Gregory P, Kraemer E, Zürcher G, Gentinetta R, Rohrbach V, Brodbeck U, Andres AC, Ziemiecki A, Bütikofer P.
|
| Title |
GPI-specific phospholipase D (GPI-PLD) is expressed during mouse development and is localized to the extracellular matrix of the developing mouse skeleton.
|
| Journal |
Bone
|
| Abstract |
Glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) is abundant in serum and has a well-characterized biochemistry; however, its physiological role is completely unknown. Previous investigations into GPI-PLD have focused on the adult animal or on in vitro systems and a putative role in development has been neither proposed nor investigated. We describe the first evidence of GPI-PLD expression during mouse embryonic ossification. GPI-PLD expression was detected predominantly at sites of skeletal development, increasing during the course of gestation. GPI-PLD was observed during both intramembraneous and endochondral ossification and localized predominantly to the extracellular matrix of chondrocytes and to primary trabeculae of the skeleton. In addition, the mouse chondrocyte cell line ATDC5 expressed GPI-PLD after experimental induction of differentiation. These results implicate GPI-PLD in the process of bone formation during mouse embryogenesis.
|
| Volume |
37(2)
|
| Pages |
139-47
|
| Published |
2005-8-1
|
| DOI |
10.1016/j.bone.2005.04.015
|
| PII |
S8756-3282(05)00146-8
|
| PMID |
15946906
|
| MeSH |
Animals
Bone and Bones / embryology
Bone and Bones / metabolism*
Cell Differentiation
Cell Line
Chondrocytes / cytology
Chondrocytes / metabolism
Extracellular Matrix / metabolism*
Immunoblotting
Immunohistochemistry
Mice
Phospholipase D / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
|
| IF |
4.147
|
| Times Cited |
7
|
|
WOS Category
|
ENDOCRINOLOGY & METABOLISM
|
| Resource |
| Human and Animal Cells |
ATDC5(RCB0565) |
