Reference - Detail
| RRC ID | 42395 |
|---|---|
| Author | Bidel S, Mustonen H, Khalighi-Sikaroudi G, Lehtonen E, Puolakkainen P, Kiviluoto T, Kivilaakso E. |
| Title | Effect of the ulcerogenic agents ethanol, acetylsalicylic acid and taurocholate on actin cytoskeleton and cell motility in cultured rat gastric mucosal cells. |
| Journal | World J Gastroenterol |
| Abstract |
AIM:To assess the effects of ulcerogenic agents on actin cytoskeleton and cell motility and the contribution of oxidative stress. METHODS:Rat gastric mucosal cell monolayers were cultured on coverslips. The cells were exposed, with or without allopurinol (2 mmol/L), for 15 min to ethanol (10-150 mL/L), ASA (1-20 mmol/L) or taurocholate (1-20 mmol/L), then the cells were processed for actin and vinculin staining. Cell migration after wounding was also measured. RESULTS:Exposure to 10 mL/L ethanol caused divergence of zonula adherens-associated actin bundles of adjacent cells and decreased rate of migration. These actions were opposed by xanthine oxidase inhibitor allopurinol. Exposure to 50 mL/L ethanol induced degradation and divergence of zonula adherens-associated vinculin from adjacent cells, which was, again, partially reverted by allopurinol. With 1 mmol/L ASA actin filaments became shorter and thicker. However, higher concentrations (10, 20 mmol/L) of ASA returned microfilaments thinner and longer, and decreased rate of migration. Zonula adherens-associated actin bundles were moderately distorted with 10 mmol/L ASA and with 10 mmol/L taurocholate. Exposure to taurocholate provoked changes resembling those of ASA. Taurocholate 5-20 mmol/L decreased the rate of migration dose dependently. The effects of ASA and taurocholate were not prevented by allopurinol. CONCLUSION:All ulcerogenic agents decreased the rate of migration dose dependently and induced divergence of zonula adherens-associated actin bundles of adjacent cells. In addition, ethanol and ASA caused degradation of actin cytoskeleton. Oxidative stress seems to underlie ethanol, but not ASA or taurocholate, induced cytoskeletal damage. |
| Volume | 11(26) |
| Pages | 4032-9 |
| Published | 2005-7-14 |
| DOI | 10.3748/wjg.v11.i26.4032 |
| PMID | 15996028 |
| PMC | PMC4502099 |
| MeSH | Actins / metabolism* Animals Aspirin / toxicity* Cell Movement / drug effects Cells, Cultured Cytoskeleton / drug effects* Ethanol / toxicity* Gastric Mucosa / drug effects Gastric Mucosa / metabolism Gastric Mucosa / pathology* Rats Stomach Ulcer / chemically induced* Taurocholic Acid / toxicity* |
| IF | 3.665 |
| Times Cited | 4 |
| WOS Category | GASTROENTEROLOGY & HEPATOLOGY |
| Resource | |
| Human and Animal Cells | RGM1(RCB0876) |